Positive Health News

Report No 18 Spring Issue (1999)

A Consumer’s Guide to Immune Restoration

The Search for “Th1”

Mark Konlee

The search for answers on how to restore a balanced and functional immune system, in the face of chronic viral challenge, seems, at times, almost as elusive as the Holy Grail, the cup from which Jesus drank wine at the last Supper. Persons affected by HIV, CFIDS, Candidiasis, Multiple allergies, Multiple Chemical Sensitivities (MCS), viral hepatitis, Pappilloma and other chronic viral infections, Gulf War Syndrome (GWS) and cancer are all affected by chronic immune dysfunction or dysregulation and are searching for answers. The subject of immunology is complex and a challenge to understand not only for the lay person, but the professional as well. Since the onset of AIDS, most research has focused on anti-viral therapies while immunology has taken a back seat. Now, even virologists realize that both areas of science are needed. In chronic conditions, there is a growing realization that immunology may provide long term answers without side effects.

Treatments from vaccines to cytokine manipulation are part of the science of immunotherapy. Cytokines control our immune responses to cancer, viral, fungal and bacterial infections. Nutrients and many other factors can induce or suppress specific cytokine responses.

What are Cytokines?

Cytokines are chemical messengers that control immune responses. They are secreted by white blood cells, T cells and epithelial cells and some other body cells. There are at least 17 different kinds of interluken and 3 classes of interferon called alpha, beta and gamma and various subsets. Interlukens and interferons are called “cytokines” and there are two general groupings, Th1 and Th2. Th1 (T-cell Helper type 1) promote cell-mediated immunity (CMI) while Th2 (T-cell Helper type 2) induce humoral immunity. In HIV progression to AIDS, there is a shift from Th1 (cellular immunity) to the less effective Th2 (humoral immunity).

Research on Cytokines

Th1 or Th2?

John Sexton of Hawaii sent me over 300 scientific abstracts on factors that affect either Th1 or Th2 cytokines. John has poured and sifted through thousands of peer-reviewed articles to create his files. His original inspiration to find and categorize all the factors that turn on these two arms of the immune system came from a magazine he discovered in 1998. The publication was Positive Health News, Report No 14, (1997) that contained an article on “Cytokines and Cellular Immunity,” by Robert Darga MD.

In his article, Robert Darga MD states:

“Two different methods exist by which the body fights infections: humoral immunity (Th2) results in the production of antibodies to neutralize foreign invaders outside of the cells, while cellular immunity (Th1) directs Killer T-cells (CD8) to attack microorganisms or abnormal cells at the sites of infection inside the cells..”

Th1 Cytokines

Darga identifies important Th1 cytokines as Interluken-2 (IL-2), Interluken 12 (Il-12) and gamma interferon (IFN-gamma).

Editor’s Note: To Darga’s Th1 list, I will add IgA (Immunoglobulin type A) that supports mucosal immunity.

Th2 Cytokines

On humoral Th2 cytokines, Darga states “The humoral immune response is enhanced by...Interlukens 4, 5 6 and 10 and alpha interferon.” He adds: “In many cases, an infection is fought with both arms of the immune system; at other times only one is needed to control the infection.”

On AIDS, he states:“As HIV infection progresses from the asymptomatic stage to advanced disease, the immune response appears to shift from the more effective Th1 state to the ineffective Th2 state.”

A failure of the Th1 arm of the immune system and an overactive Th2 arm is implicated in a wide variety of chronic illnesses. These include AIDS, CFIDS, Candidiasis, Multiple allergies, Multiple Chemical Sensitivities (MCS), viral hepatitis, Gulf War Syndrome (GWS) and cancer and this list is by no means complete. If these two arms of the immune system could be balanced by stimulating Th1 and decreasing Th2, then many of the symptoms associated with these chronic illnesses would diminish or disappear and we would have found the answer to immune restoration and balance or the equivalent of a cure.

The Need for Balance

It is important to realize that a normal functioning immune system needs both arms - Th1 and Th2 to provide flexibility to respond to different kinds of pathogens (viruses, fungi, mycoplasmas and bacteria etc) both inside and outside of the cells. The Th2 arm becomes “bad” only in the sense that in many chronic disease states, viral, candidiasis etc. and cancer, the Th2 arm is chronically overactive while the Th1 arm is underactive. In end stage illnesses, both arms of immune system fail.

IL-12 & IgA for Mucosal Immunity

The skin outside the body and the mucus membranes inside are nature’s bubble or protective envelope to keep out unwanted pathogens. An open cut and/or a leaky gut with small pin holes in it is like a fortress with an open door. The enemy (viruses, fungus bacteria, parasites, etc) have easy access to get inside. Il-12 stimulates a CD8 Killer T cell response and the Killer T cells (cytotoxic lymphocytes) in the mucus membranes stop viral invasions before they get inside the body. Restoring normal IgA in the mucus membranes is also critical to help reduce excessive IgE (linked to food and chemical allergies) and to restoring mucosal immunity - a critical first step to take in immune restoration.

Scientific research has established that the mucus membranes of the intestines are a site for very active HIV replication (and the destruction of CD4 cells), candidiasis, cytomegalovirus (CMV) and other pathogens.

(See related article elsewhere in this newsletter “HIV primarily infects and destroys CD4 cells in the intestines.”).

The epithelium (mucus membranes) of the intestines are a fine filter to let nutrients in and keep out unusable food particles. When pathogens like HIV, candida albicans, etc., replicate in the mucus membranes, they eat away at structure of the epithelium and create tiny pin holes that allows byproducts of faulty digestion to enter the blood. This triggers an antibody response (Th2) that weakens the cell-mediated immune response systematically (Th1).

MUCOSAL IMMUNITY = NATURAL IMMUNITY AGAINST HIV/AIDS AND OTHER SEXUALLY TRANSMITTED DISEASES (STDs)

Study after scientific study has found that in persons who have natural immunity to HIV infection after multiple exposures through unprotected sex have had high levels of IgA and CD8 Killer T cells in the mucus membranes of the colon or/and vagina. To increase the CD8 Killer T cells in the mucus membranes and throughout the body (systemic), we will need to induce more Interluken-12 (IL-12). IgA and IL-12 along with interferon-gamma (IFN-gamma) are the key cytokines for restoring mucosal and systemic cellular immunity.

DIETARY FACTORS THAT INCREASE Th2 CYTOKINES

The three most common factors that drive Th2 cytokine responses are: 1. Faulty digestion leading to absorption of partially digested and unusable proteins and other food particles (increases IgG and IgE antibody responses that are directed against these foreign food particles). Here we see the therapeutic benefits of digestive enzymes, eating slowly mixing lots of saliva with food and eating only when hungry).

2. White sugar and glucose and all processed foods containing these (Coke, canned soda, candy bars, cake, pie, sweet rolls etc.) - directly weakens the functioning of macrophages, natural killer cells and other white blood cells and weakens systemic resistance to all infections.

3. Consuming trans-fatty acids found in most heated and processed vegetable oils (soy, canola, safflower, corn and sunflower) that are high in n-6 fatty acids (linoleic) and food products made with them (i.e. french fries and potato chips). Vegetable oils high in linoleic trans-fatty acids stimulate IL-6 and depress delayed type hypersensitivity (DTH) thus weakening CD8 Killer-T cell activity. The trans-fatty acids are twisted out of their normal “cis” shape and produce cell membranes that are porous and vulnerable to viral infections.

For example, persons who consume canned soda (i.e. Coco-cola) and french fries daily and are already immune compromised will worsen their condition by these dietary choices. Many other factors that stimulate a Th2 response will be discussed later. Some herbs (Echinacea and Astragalus) stimulate both Th1 and Th2 cytokines.

DIETARY FACTORS THAT SUPPORT Th1 CYTOKINES

Omega 3 fatty acids (N3) found in cold water fish reduce IL-6, tumor necrosis factor and support DTH. Oleic acid, found in olive oil (cold pressed) and hazelnut oil, vitamin A and L-glutamine increases IgA and improve mucosal integrity. The use of two heat-treated strains of lactobacillus - L. Plantarum and L. Casei induces a strong IL-12 response and increases IFN-gamma and strengthen the systemic immune response against all intra-cellular infections and even cancers. The intestinal flora Bifidobacterium longum also increases IgA and improves mucosal immunity. Silica reduces excess IgG and this improves NK function and improves the integrity of the skin and mucus membranes.

1. Omega 3 fatty acids (DHA/EPA) found in all cold water fish, especially in salmon, sardines, mackerel, halibut and trout. Supplements: Max DHA (Jarrow Formulas) Salmon oil capsules, Cod liver oil. DHA - 1500 to 2500 mg daily and EPA - 500 to 1000 mg daily.

2. Oleic acids (Monounsaturated). Best choice is cold pressed olive oil (a therapeutic dose is 4 tablespoons daily), 2nd best - hazelnut or filbert oil. Also green and ripe olives, filberts and hazelnuts. Third - almonds and almond oil.

3. Vitamin A - 2 tablespoons of cod liver oil daily (Dale Alexander emulsified) or 25,000 i.u. daily of Vitamin A with vitamin D plus 1/2 to 1 lb daily of any of the following sourcesof carotenoids: cooked carrots, squash, pumpkin and sweet potatoes (yams).

4. L-Glutamine - 10 to 20 grams or more daily or as directed. Maintenance: 2000 mg daily.

5. Silica - One serving of cooked oatmeal or millet daily and/or the herb horsetail (3 caps 2X) or Bio-Sil (Jarrow Formulas) - the most bio-available form - 10 drops 3 times a day. Maintenance - 10 drops once a day.

6. L-plantarum and/or L casei and B. longum - up to 1 teaspoon of powder 2 or 3 times a day. After 7 to 14 days of use, you may only need to use this 3 days per week.

Other products like Neem, soil based organisms (SBO’s) and supplemental factors that replace glutathione help Th1 responses. The more of the above items you use, the faster you will see results. It is critically important to completely avoid processed vegetable oils and foods cooked with them and other factors that promote Th2 cytokines.

LONG TERM SURVIVORS AND Th1

Anthony Fauci and IL-2

Darga reports that

“Cellular killing of HIV-infected cells may be at the heart of the cellular immune response which seems so effective in long-term survivors.”

Fauci has publicly commented about studies being done at the NIH where injectable IL-2 is being given to persons on HAART or triple combo drug therapy for HIV that has reduced their plasma viral loads to non-detectable levels. Fauci thinks it may be plausible that Il-2 could flush out the HIV virus hiding in reservoir cells such as the lymph nodes and macrophages and the drugs then will finish killing off the virus. While a few patients have gone to non-detectable levels in some of these reservoirs, not all the cellular reservoirs have been tested, so it remains uncertain if IL-2 therapy combined with protease inhibitors will lead to total viral eradication. So far, total viral eradication hasn’t happened.

What has occurred in several cases is that some persons have gone off their drug regimens and their plasma viral loads have remained non-detectable, even though small amounts of the virus harbor in the reservoirs of the lymph nodes and other areas. What is now being reported in the Wall Street Journal (Jan 25, 1999) is the probably that the immune system of these patients now controls the virus without the need to take further drugs. The article mentions the Berlin patient who has been off the drug cocktails for 18 months and still has a non-detectable viral load. This immune response is thought in most quarters to be coming from Killer T cells aka CD8 Cytotoxic Lymphocytes (CD8 CTL’s).

In the search for an AIDS cure, the NIH reportedly plans to spend 35 million on Il-2 research. Can Il-2 do this alone? Is the NIH placing too much emphasis on this one cytokine? How about the other Th1 cytokines, IL-12, IFN-gamma and IgA? Would not the goal of immune restoration that may lead to total viral eradication be attained faster if these three were combined with IL-2?

Interferon-gamma blocks HIV-1 and HIV-2 infection in human colon epithelial cells

France: Tantini, Yahi and de Micco reported that human cells from the mucus membranes of the colon (large intestines) called HT29 cells, pretreated with gamma interferon at 100 U/ml, was sufficient to block their infection with HIV-1 and HIV-2 isolates. They concluded that

“IFN-gamma, at a concentration compatible with clinical use, has a potent anti-HIV effect on human colon epithelial cells. Therefore, this cytokine should be tested for the prevention and treatment of gastrointestinal disorders with HIV-1 or HIV-2 infection.” (1) 1. Int AIDS conf. 1993 Jun 6-11;9(1):171 (abstract no PO-A13-0221)

In my opinion, it is safer to stimulate the body’s own production of Th1 cytokines than to take them by injection from an outside source. For the past two years, I have been looking for substances that would stimulate the body’s own production of IL-12 and IFN-gamma. Il-12 is a key cytokine known to activate CD8 cytotoxic lymphocyte (CTL) activity. The good news is that scientific research has identified several substances that can increase Th1 cytokines including IL-12 and IFN-gamma. Substances that stimulate Th2 cytokines have been identified and can be avoided.

Inducing Th1 cytokines

Omega-3 Fatty Acids

Fish oils containing omega 3 fatty acids improve cell mediated immunity (CMI) and reduce IL-6, TNF, triglycerides and increase DTH while soybean oil and most vegetable oils used at high temperatures produce toxic trans-fatty acids that suppress immune function.

Following trauma, injury or invasion of the body by pathogens, pro-inflammatory cytokines, Interluken 1 and 6 and tumor necrosis factor (TNF), are rapidly produced in response to the injury. However, when this proinflammatory response fails to shut down, pathological (damaging) effects result.

Switzerland: Researchers Grimble and Tappia report in a review medical journal (1)

“excessive production of pro-inflammatory cytokines, or production of cytokines in the wrong biological context, are associated with mortality and pathology in a wide range of diseases, such as malaria, sepsis, rheumatoid arthritis, inflammatory bowel disease, cancer and AIDS.....Among the nutrients, fats have a large potential for modulating cytokine biology. A number of trials have demonstrated the anti-inflammatory effects of fish oils, which are rich in n-3 polyunsaturated fatty acids, in rheumatoid arthritis, inflammatory bowel disease, psoriasis and asthma..”

The n-3 fatty acids are known as the Omega 3 fatty acids and are abbreviated as DHA and EPA. These oils are found in high amounts in salmon, sardines, mackerel and trout and many other cold water fish. In contrast, n-6 fatty acids (Linoleic) are found primarily in vegetable oils (i.e. soybean , canola etc).

In another article by Grimble, he states:

“Monounsaturated fatty acids and omega-3 ...fatty acids suppress TNF and IL-1 production and actions, while n-6 (polyunsaturated vegetable oils) exert the opposite effect.” (4).

In France, researchers Khalfoun et al found that the addition of n-3 type fish oils (DHA/EPA) reduced IL-6 production in endothelial cell lines. They report that the n-3 fatty acids from fish oils suppress inflammation. (2)

Japan: Tashiro et al report that patients fed soybean oil after surgery had an increase in interluken 6 production while patients fed EPA (found in fish oil) had a decease in interluken 6 production and improved cell-mediated immunity 3 weeks after operation. Tashiro also reports that

“DTH, granulocyte-macrophage colony-stimulating factor and EPA content increased proportionally with the intravenous dose of fish oil emulsion.”(3)

In his book, “Fats that Heal - Fats that kill,” Udo Erasmus reports that DHA and EPA (from fish oil) reduced triglycerides by 65% in one study. Erasmus also reports that EPA and DHA keep platelets from sticking, lowers fibrinogen levels in the blood and prevents hardening of the arteries, prevents strokes and inhibits the growth of cancer and tumors. (5) Erasmus also advises avoiding fish that contain cetoleic acid as it is a difficult fatty acid for the body to break down. Cetoleic acid is found in herring, capelin, menhaden and anchovetta. (5)

References:

(1)Modulation of pro-inflammatory cytokine biology by unsaturated fatty acids. by Grimble RF, Tappia; Z Ernahrungswiss 1998;37 supple 1:57-65

(2)DHA and EPA acids inhibit in vitro human endothelial production of interluken 6. Khalfoun et al, Adv Exp Med biol 1997; 400B:589-97

(3). n-3 versus n-6 polyunsaturated fatty acids in critical illness. Tashiro et al; Niutrition 1998 Jun; 14(6):551-3.

(4). Nutritional modulation of cytokine biology, Grimble RF; Nutrition 1998 Jul-Aug;14(7-8):634-40.

(5). Fats that Heal, Udo Erasmus; Alive Books, Vancouver, Canada.

Conclusion: Most vegetable oils should be avoided as they suppress cell-mediated immunity (CMI), the exception are those vegetable oils high in monounsaturated fatty acids like extra virgin olive oil, hazelnut oil etc. Almond, avocado or peanut oil are also high in monounsaturated oils and could safely be used in moderation.

GOOD ESSENTIAL OILS - DHA/EPA

The Essential Oils “Omega-3” (DHA/EPA) are very beneficial and therapeutic for persons with a predominant TH2 cytokine profile who need more support for a TH1 cytokine profile. This includes persons affected by HIV, HHV-6A, CFIDS, FMS, multiple allergies, candidiasis, GWS, MCS, CMV, irritable bowel syndrome, EBV, psoriasis, heart disease and cancer.

1. DHA/EPA: Salmon, sardines, mackerel halibut and trout have the highest concentrations of DHA/EPA as these types of cold water fish have a high fat content. Lesser amounts of DHA/EPA are found in all varieties of cold water fish. Small amounts of DHA/EPA are found in tropical fish and in dark-green leafy vegetables and sea vegetables (blue green algae, chlorella etc.).

Tuna fish is high in DHA/EPA but is also high in mercury, a toxic heavy metal. Tuna should be used less frequently (once a week or less) than other types of fish. It is better to consume canned, boiled or broiled fish rather than fried fish. The vegetable oils often used in frying fish will promote a TH2 cytokine response that will negate the benefits of the good oils in fish that support the TH1 response.

Note: If you find the taste of olive oil objectionable when pan frying fish, try adding herbs or cook with a mixture of coconut oil and butter or hazelnut oil and butter. However, give olive oil a chance. I personally found it tasted excellent in a pasta dish topped with cooked lean ground sirloin and tomato sauce. The pasta was made from brown rice.

Supplements: Max DHA (Jarrow Formulas - consider 2 or 3 capsules twice a day), Salmon oil capsules in light proof capsules (2 or 3 capsules twice a day). Great choices - canned salmon, especially red sockeye, sardines canned in water or olive oil (not soy oil), broiled or boiled trout, halibut or mackerel. Three servings a week are recommended for maintenance. For therapeutic purposes, a daily serving is suggested.

(Note: Fresh ground flaxseed contains a small amount of N-3 fatty acids. One or two tablespoons of freshly ground flaxseed mixed with apple sauce is beneficial on a daily basis and increases oxygen availability to the cells and helps lessen fatigue).

2. Monounsaturated oils - Extra Virgin Olive oil, hazelnut oil that are both very high in monounsaturated fats. Best used raw, but may be used in moderation for cooking. For salad dressing, use Extra Virgin Olive oil or hazelnut oil. For baking purposes, best choices are coconut oil, butter or hazelnut oil. You can use almond oil.

THE NEUTRAL OILS -

neither suppress nor enhance cell mediated immunity - coconut oil. May be used alone or mixed with butter for cooking purposes.

THE BAD OILS - HYDROGENATED VEGETABLE OILS OR LIQUID VEGETABLE OILS USED AT HIGH TEMPERATURES PRODUCE TOXIC TRANS-FATTY ACIDS.

Hydrogenated vegetable oils used in making margarine and shortening should be strictly avoided as these contain trans-fatty acids. Trans-fatty acids produce cell membranes that are defective; that is, they have small holes in the membranes as the fats do not fit together like a tightly knit puzzle. Trans-fatty acids are twisted out of shape by heat and weaken the membranes of individual cells making them porous and vulnerable to viral infection.

In his book “Fats that Heal and Fats that Kill, Udo Erasmus states:

“Besides producing atheroclerosis in the arteries of animals, fried and deep fried (vegetable) oils can also impair cell respiration and other cell functions, inhibit immune functions, and lead to cancer.”

(1) Soybean oil, safflower, corn, sunflower, canola and most other vegetable oils should be avoided by persons with chronic illness who have a predominant TH2 cytokine profile (too much Il-6, Il-10, TNF and triglycerides). It is nearly impossible to find vegetable oils that are strictly cold processed. Except for expeller pressed oils, nearly all other vegetables are processed with solvents, hexane or gasoline, to remove the oils from the seeds. The oil and hexane/gasoline mixture is then heated to 302 degrees F. to remove the hexane or heptane (gasoline) (1). At this point, the oils are now toxic for human use as they are twisted out of their natural “cis” shape and become trans-fatty acids. Add heat, light and oxygen to oils and the lipid peroxides increase and they become rancid and develop a strong taste. The safest and freshest oils to buy are cold pressed (best choice) or expeller, that are stored in a can or black bottle to keep out light. It is even better if Vitamin E or BHT is added as an antioxidant to prevent lipid peroxide formation.

1. Fats the Heal/Fats that Kill, Udo Erasmus. Alive Books, Vancouver, Canada.

L-Casei and L. Plantarum - Lactobacillus that promote IL-12 and IFN-gamma

Of all the strains of lactobacillus that are available including acidophilus, two strains stand out as exceptional stimulators of Th1 cytokines. They are L-Casei and L. Plantarum. Both strains of intestinal flora strongly increase Il-12 production along with gamma interferon thus increasing CD8 cytotoxic lymphocyte activity against most kinds of intracellular viral infections.

L. Plantarum - potent inducer of IL-12

Seven Researchers from Japan, Murosaki S et al, report in the July, 1998, Journal of Allergy and Clinical Immunology (1) that “Heat-killed Lactobacillus plantarum L-137 suppresses naturally fed antigen-specific IgE production by stimulation of IL-12 production in mice.” They report that L. Plantarum directly increased Il-12 and IFN-gamma in mice in vitro in peritoneal macrophages and spleen cells. They report that production of IgE against dietary antigens is a common cause of food allergies and that L plantarum by increasing IL-12 suppresses IgE production. They obtained these effects with heat-killed L plantarum.

They also found that IgE can be suppressed by directly giving IL-12 to mice. They also report that giving L plantarum to mice in vivo (in the living mouse) increased plasma levels of IL-12 and that Il-4 (a Th2 cytokine) was suppressed. Their research found that L plantarum increased IL-12 production in mice in both vitro and vivo (in the lab and in the mouse). They concluded that L-plantarum is “a potent inducer of IL-12, is useful for the prevention and treatment of food allergy.”

1. Murosaki S et al; J. Allergy Clin Immunol 1998 Jul;102(1):57-64

L. Casei - induces IL-12, IL-2 & IFN-gamma - suppresses IL(4,5,6 and 10) anti(viral & bacterial), anti-cancer effects

Japan: Matsuzaki T has found that in mice given the Lactobacillus Casei strain, that it induced the production of several cytokines including IFN-gamma, IL-beta and TNF-alpha, resulting in the inhibition of tumor growth and chemically-induced bladder cancer. Matsuzaki states that L. Casei

“has the potential to ameliorate or prevent a variety of diseases through modulation of the host’s immune system, specifically cellular immune responses.”(1)

Other Japanese researchers, Kato, Endo and Yokojura found that at a dose of 100 to 200 mg/kg/day of L. Casei for 7 days, implanted tumor growth was suppressed, but not in the control mice who did not receive this strain of lactobacillus. In this experiment, live L. Casei, rather that heat-treated, was used. (2)

In another experiment, Matsuzaki, Yamazaki, Hashimoto and Yokojura tested heat-treated L Casei for its effects on IgE levels in mice. The L-Casei was given orally. Elevated IgE have been linked to food allergies by many researchers. What the researchers found was that L. Casei, like L. Plantarum, inhibited IgE production in the mice receiving L. Casei who were immune challenged with ovalbumin. There was no inhibition of IgE in the control mice receiving only ovalbumin.

They also reported an increase in the production of IL-2 and IFN-gamma in the mice receiving L. Casei and equally important, a reduction in the Th2 cytokines - IL-4, IL-5, IL-6 and IL-10 as compared to controls! They also reported that IL-12 in the spleen cells of the mice fed L. Casei was higher than in the controls. (3)

Shimizu et al found that L. Casei also induced colony-stimulating factor in mice and found that the macrophages showed strong anti-tumor activity in two groups of mice tested. They reported that the benefits of L Casei could be negated by pretreating the mice with carrageenan which suppresses macrophage function. They found these effects both in vivo and in vitro. (4)

Saito et al reports that heat-killed L. Casei (strain 9018) was studied for its protective effects against Mycobacterium fortuitum complex and M. chelonae infections in mice. They injected the L. Casei into the mice and reported an increase in macrophage activity and phagocytic function. They thought this might explain the protective and therapeutic efficacy of L. casei against infection due to Mycobacterium fortuitum complex (5).

L-Casei vs. Cytomegalovirus (CMV)

Ohashi et al report that heat-killed L. Casei was effective against CMV in mice and protected them against a lethal dose of CMV in contrast to controls that did not receive the lactobacillus casei. (6). Other researchers, Watanabe T and Yamori T, have found that heat-killed L Casei increased cytotoxic activity against cells from mice infected with herpes simplex virus. (7)

L. Casei or B. animalis inhibit Candida Albicans

Research from the University of Wisconsin by Wagner Rd et al has shown that L. Casei and Bifidobacterium animalis (B animalis) significantly reduced candida albican colonization in the intestines of immune deficient mice. (8) References:

1. Int J Food Microbiol 1998 May 26;41(2):133-40

2. Int J Immunopharmacol 1994 Jan;16(1):29-36

3. J Dairy Sci 1998 Jan;81(1):48-53

4. J Leukoc Biol 1987 Sep;42(3):204-12

5. J. Gen Microbiol 1987 Oct;133(Pt 10):2843-51.

6. Biotherapy 1998;1(1):27-39

7. Kansenshogaku Zasshi 1989 Mar;63(3):182-8

8. Infect Immun 1997 Oct;65(10):4165-72

ADVERSE EFFECTS OF LIVE L. CASEI IN AN AIDS PATIENTS WITH NO T CELLS

Why only heat-killed L. Casei should be used in persons severely immune compromised.

I read two reports, one from the Lancet, on AIDS patients with no CD4 cells, where L. Casei got into the lungs and caused pneumonia. The problem can happen with someone with leaky gut syndrome where good intestinal flora like L casei pass through the mucus membranes of the gut and get into the blood stream. The same thing often happens with candida albicans that get into the blood stream and cause systemic candidiasis. There is no problem with the single cell candida albican as long as it stays in the gut area. The problem is when the integrity of the mucus membranes breaks down due to chronic viral or fungal infections and that only occurs when there is a failure of mucosal immunity. Research also shows that when candida albicans colonize in the gut membranes that there is little or no IgA present.

Are there any species of intestinal flora that promote Th2 cytokines?

Answer: Yes - “streptococcus thermophilus” - widely used in the making of commercial yogurt.

Marin ML et al from Michigan State University found that streptococcus thermophilus significantly increased IL-6 production in macrophage lines. They also found that lactobacillus bulgaris and B. bifidum also increased IL-6, although less than S. thermophilus. (1)

For some time, I have been puzzled by reports from persons with CFIDS or chronic candidiasis who say they feel worse when they eat yogurt. While most strains of commercial yogurt add acidophilus, many also have thermophilus and that is not shown on the label. Could it be that the S. thermophilus strain was in the yogurt they consumed, increasing IL-6 and worsening their symptoms? Perhaps eating yogurt may not be a good idea after all even though research shows that acidophilus inhibits tumor growth in laboratory animals.(2) If the yogurt contains more thermophilus than acidophilus, then there will be a net gain of Th2 cytokines and a loss of the Th1.

In choosing products to stimulate cytokine production, the choices may not always be clear due to cross regulatory effects (Th1 or Th2) so the positive and negatives have to first estimated first before making a choice.

1. J Food Prot 1998 Jul;61(7):859-64.

2. Nutr Cancer 1997;28(2):130-4

Bifidobacterium longum increases IgA supports mucosal immunity

Takahaksi T et al report on mice fed Bifidobacterium longum (B. longum) in a controlled experiment to determine its effects of IgA in the intestines. They found that B. longum significantly increased IgA in the small intestines and in the peyer’s patches. They suggest the B longum may protect the intestinal mucosa from dietary antigens that have escaped digestion by enzymes. (1) Other research shows that B. longum can help persons overcome lactose intolerance (2).

1. Biosci Biotechnol Biochem 1998 Jan;62(1):10-5

2. J Dairy Sci 1996 May;79(5):75-7.

JAMA COMMENT

The Journal of the American Medical Assn (JAMA) commented in a July, 1996, article on the therapeutic uses of intestinal flora. After reviewing all the published material since 1966 on the subject, they reported that controlled studies have established that L. casei, L. acidophilus, B longum and Saccharomyces boulardii have been successfully used to treat infantile diarrhea caused by antibiotics. They said the time has come to explore the therapeutic applications of these agents.(1)

1. JAMA 1996 Mar 20;275(11):870-6

WHERE TO FIND L. CASEI, L. PLANTARUM AND B. LONGUM

As of the publication of this edition of Positive Health News, I have been unable to find a source for the separate isolated strains L casei, L. plantarum and B longum. What I have found is that the Jarrowdophilus formula contains the following:

L. casei - 20%

L. plantarum - 20%

L. rhamnosus - 20%

L. acidophilus - 10%

B. longum - 10%

B. breve - 10%

B. bifidum - 10%

Of the 7 strains, the most beneficial ones that published literature indicate support Th1 cytokines are L. casei. and L. plantarum (IL-12 and IFN-gamma), L. acidophilus (IL-2) and B. longum (IgA) and these comprise 60% of the formula. Fortunately, there is no streptococcus thermophilus in the formula that strongly activates IL-6, something we do not want.

Overall, the Jarrowdophilus product should strengthen Th1 cytokines until Jarrow Formulas or another source makes available a formula that exclusively supports Th1 cytokines or if we can find a source of the isolated strains sold separately.

HOW MUCH JARROWDOPHILUS WOULD YOU TAKE TO OBTAIN AN EFFECTIVE DOSE?

Jarrowdophilus is available in capsules and in powder form (70.5 grams or 1000 gram bottles). Since the quantity needed may be significant, it would be more cost effective to use the powder than the capsules.

Since research has been limited to mice, it is possible that an effective dose would be less in humans than the amounts suggested in animal studies (100 mg /kg). Certainly, it would be prudent to start off with a small dose and gradually increase it. For starting off, I would use about 1/4 teaspoon (about 625 mg) three times a day and gradually increase in increments of 1/4 teaspoon to eventually reach 1 teaspoon 2 or 3 times a day (5 to 7.5 grams daily). I would start off with 1/4 tsp. 3 times a day for 3 days before increasing it to 1/2 tsp. 3 times a day. This gives you time to observe your own reactions to the increasing dosage levels. If you feel the dose is too strong, back off; if too weak, increase it.

In measuring quantities, one teaspoon of powder contains about 2500 mg.

SHOULD YOU HEAT-TREAT THE LACTOBACILLUS or USE IT RAW?

The idea of “heat-treated” friendly flora is contrary to everything that we have ever been told. However, with beta glucan that comes from the common yeast, would anyone want to eat live baker’s yeast as a source? No, because live yeast may pass through those pin holes in your gut and end up in your blood where they will further stress the Th2 humoral antibody response. It is the membrane structure of the yeast that we want and where the beta 1,3 D glucan can be found. One abstract identified “glycoproteins” in the membranes of L. casei as the substance that possibly activates the IL-12 and CD8 CTL response. These glycoproteins, as immune stimulants, are part of the membrane of the lactobacillus and are not some thing produced by the lactobacillus. Interestingly, HIV glycoproteins being tested in some vaccines are reported to do the same thing - activate the cytotoxic lymphocyte response against HIV and other viruses. However, not everyone agrees. One Japanese researcher claimed it was the live, raw L casei and not the heat-treated that activated anti-tumor responses. Other researchers used heat-treated L casei and both sides claim to be getting results.

The only problem with live L casei would be in a person with leaky gut syndrome and who is severely immune-compromised where the lactobacillus would pass into the blood and start growing in an area of the body where it is not supposed to be. In that case, research has shown that it could also cause platelet aggregation increasing the risk factor for a blood clot. For persons with more intact immune systems, the live or raw lactobacillus would, in my opinion, be the best choice. Since the heat-treated lactobacillus strains of casei and plantarum have been demonstrated to induce IL-12 and IFN-gamma, the use of the heat-treated strains would eliminate those risk factors.

The vice-president of Jarrow Formulas, Charles Fisher, told me that adding the powder to a 1/2 cup of water and bringing it to a boil for one minute would certainly inactivate the lactobacillus. In testing this out, I found that you need to stir it in the cold water with a fork or whisk to dissolve it before bringing it to a boil. An attempt to make yogurt with it failed after this treatment indicating inactivation had occurred.

You could also add the Jarrowdophilus to cold water before making breakfast cereal and cook it together with the cereal.

(Th1) GINSENG vs. CANCER

Researchers in Korea have found that an acidic polysaccharide (ginsan) derived from Panax ginseng promotes TH1 cytokines. Kim KH et al state:

“Spleen cells became cytotoxic to a wide range of tumor cells after 5 days of culture with ginsan in a non-major histocompatibility restricted manner and the activity of ginsan was 12 times higher than that of lentinian.... ginsan induced LAK cells were CD8+- cells...ginsan induces the expression of mRNA for IL-2, IFN-gamma, IL-1 alpha, and GM-CSF....ginsan generates LAK cells from both NK and T cells...This property may contribute to its effectiveness in the immunoprevention and immunotherapy of cancer.” (1)

1. Kim KH et al; Planta Med. 1998 Mar;64(2):110-5

RED KOREAN GINSENG (KRG) VS HIV

Results of a long-term study

Korea: Cho YK, Lee, Oh and Kim report on a 53 month study using 5.4 grams of KRG daily on 16 HIV+ patients from 40 to 57 months (average - 53 months which makes this a 4 year plus study. A control group of 10 that took no antiretroviral drugs and did not use KRG was followed as a comparison. In the group using KRG, the average CD4 count at baseline was 301. After 53 months, the average CD4 count was 359. In the control group, the average baseline CD4 count was 352. After 53 months, it was 156. In the group using KRG, the Beta2-M decreased from 2.48 to 2.21. Cho et al concluded that

“KRG has definite long-term immune modulating effect without side effects on HIV-infected patients.” (1).

In another study from Seoul, Korea, Yun and Choi found in a study of 1987 persons that ginseng users had a lower risk of developing cancer than non-users. The data they published indicates that red ginseng was more effective in preventing cancer that the Korean white ginseng. (2).

1. “Long term immunological effect of ginseng on HIV-infected patients,” Cho YK et al,; Abstr Gen Meet Am Soc Microbiol. 1997 May 4-8;97:247 (abstract no. E44)

2. “Preventive effects of ginseng intake against various human cancers”; Yun TK et al; Cancer Epidemiol Biomarkers Prev 1995 Jun;4(4):401-8.

SIBERIAN GINSENG AND PCM-4 vs. HIV

In the Sep/Oct, 1995, issue of Positively Aware, is a article on PCM-4, OTC hopeful? The magazine noted that while the manufacturer, Omega Nutripharm, is unable to make claims because of FDA restrictions, that PCM-4 reduced tumor necrosis factor (TNF). Studies have shown that high TNF levels are associated with wasting syndrome and increased HIV replication. The product is reported cost about $110 a month at buyer’s clubs.

In an abstract filed at the Int’l AIDS Conference in 1993, (abstract no PO-B29-2172), the authors reported in a study, preliminary results indicate that beta2 microglobulin levels and total immunoglobulin levels declined towards normal in over 50% of the patients. In symptomatic patients, wasting symptoms were reversed, TNF levels declined and “there was a striking lack of opportunistic infections.”

“Imperial Elixir” Siberian Ginseng is a 5 to 1 concentrate and is cost effective. Will it match the results of the KRG?

While PCM-4 is reported to cost over $100 a month to take an effective dose, I have found a low cost Siberian Ginseng Extract (SGE) in capsules that costs about $24 a month. It is a 5 to 1 concentrate, meaning that one 500 mg capsule is the equivalent of taking 5 regular Siberian Ginseng (SG) capsules of the ground herb. Taking 2 capsules twice daily of SGE is the equivalent of taking 20 regular Siberian Ginseng capsules. While this brand of Siberain Ginseng has not been tested in studies, this 5 to 1 concentrate has the potential to have therapeutic value.

Suggested dose: 2 capsules twice daily. 100 capsules list for $18.50. In contrast, Imperial Elixir Red Ginseng costs 23.50 for 100 capsules. If you need to take 10 or 11 daily, it could cost $70 or more per month to use Red Korean Ginseng. On the other hand, the Red Korean Ginseng has a successful 4 year study behind it. Would a person want to swallow 11 pills a day if you can get the same results from 2 capsules twice a day or will KRG work effectively at a lower dose? Because of the success with PCM-4 that is a concentrated extract of Siberian Ginseng, there is basis to believe similar results can be obtained at a lower cost with the Imperial Elixir Siberian Ginseng 5 to 1 concentrate.

What results to look for? Lower Beta2-M, Lower TNF, gain of lean muscle mass, stabilized CD4 counts that tend to slowly rise, stabilized viral load that tends to drop and increased physical and sexual endurance. Specific tests for Th1 - look for higher IL-2 levels and declines in IL-6 (a Th2 cytokine).

(Th1) - Chlorella

Hasegawa T and nine other researchers in Japan (1) have found that in MAIDS mice given a liquid oral extract of Chlorella and subjected to Listeria infection found that it increased “the expression of gamma IFN and IL-12 mRNA in the spleen.” They reported that in both normal mice and mice with murine acquired immunodeficiency syndrome (MAIDS) that Chlorella enhances cell-mediated immunity. They reported that chlorella augments the levels of IL-1 alpha, Il-12, GM-CSF, MIP and TNF alpha genes in the peritoneal adherent cells. They conclude that chlorella may “preferentially augment TH1 responses against Listeria via activation of macrophages to produce 1l-12 and enhance host defense against Listeria infection in both normal and MAIDS mice.” The article did not state how much Chlorella was given to the mice to enhance their cellular immunity.

Since chlorella can increase Il-12, what about spirulina and other sea vegetables? What about those persons on macrobiotic diets who ate sea vegetables daily for years and have completely stopped HIV progression? In past issues of this newsletter, I interviewed two such persons who have been long term non-progressors and who have followed a macrobiotic diet for many years that included the daily consumption of sea vegetables.

Dosage: 10 to 20 grams of Chlorella daily is suggested for adults until more is known about an effective dose. Two sources have told me that a liquid chlorella called “Wakasa” by Sun Chlorella was the most therapeutic form available. For persons buying chlorella by the bottle, I would advise against buying the tableted form as it contains binders that may interfere with absorption. Capsules or granules are far better assimilated than tablets. As a food, Chlorella can be used daily.

1. Immunopharmacology 1997 Jan;35(3):273-82

Soil Based Organisms (SBO’s) (Bacillus Subtilis and Bacillus Lichenformis) inactivate HIV, SIV and other lipid envelope viruses, along with mycoplasmas, fungus and bacteria by producing “surfactin” a lipopeptide antibiotic

Last week, a search for the word “bacillus subtilis at the National Library of Medicine (NLM) produced over 14,000 hits, indicating that the word in mentioned in over 14,000 published scientific articles. One year ago I published an article on soil based organisms and interviewed persons who had received considerable benefit from a number of long standing conditions; CFIDS, candidiasis, herpes, allergies, strep and staph infections, to name a few along with published reports that SBO’s helped shift the cytokine profile from Th2 to Th1.

The big surprise in my search is that bacillus subtilis and lichenformis produced a “detergent-like” substance called “surfactin” that dissolves the lipid membranes of lipid envelope viruses thereby rendering them completely inactivated. Surfactins’ action in dissolving lipid viral membranes is similar and probably more effective than monolaurin (a hydrolyzed from of lauric acid). Here is what German researchers Vollenbroich D et al say:

“The antiviral activity of surfactin, a cyclic lipopeptide antibiotic and biosurfactant produced by Bacillus subtilis, was determined for a broad spectrum of viruses, Semliki forest virus (SKV), herpes simplex virus (HSV-2, HSV-2), suid herpes virus (SHV-1) vesicular stomatitis virus (VSV), simian immunodeficiency virus (SIV).....Inactivation of enveloped viruses, especially herpes and retroviruses, was much more susceptible than that of non-enveloped viruses...concentrations up to 80 microM of surfactin led to a titre reduction of >4.4 logs10....for SIV and VSV in 60 min.”(1)

Japanese researchers also reported anti-HIV effects of surfactin, but gave no details. (5).

Mycoplasma and bacterial membranes also disintegrated by exposure to surfactin (3). A lipopeptide similar to surfactin is produced by bacillus lichenformis that may be even more potent at dissolving lipid envelope viruses than surfactin from subtilis (4). Potent antifungal volatiles (AFV) that inactivated most types of fungus are produce by bacillus subtilis (2).

Editor’s note: Several persons with either CFIDS or chronic candidiasis have told me of significant improvements in how they feel using “Nature’s Biotics” that contains both the bacillus subtilis and lichenformis. Now that we have substantial scientific research that demonstrates that surfactin produced by subtilis inactivates lipid-envelope viruses, persons with HIV, HHV-6 (strains A and B), EBV, CMV, herpes (all lipid envelope viruses) may want to add these strains of bacillus to their defense arsenal. Ultimately, PCR viral load testing to determine how much of a drop in viral load occurs will determine how effective SBO’s are for these different kinds of viruses.

1.Biologicals 1997 Sep;25(3):289-97

2. Fiddaman PJ et al. J. Appl Bacteriol 1994 Apr;76(4):395-4-5

3.Appl Environ Microbiol 1997 Jan;63(1):44-9

4.Appl Environ Microbiol 1994 Jan;60(1):31-8

5. Itokawa H et al, chem Pharm Bull 1994 Mar;42(3):604-7

Other products that promote Th1 cytokines

Neem- promotes IFN-gamma inhibits HIV-1 and candida albicans

Neem is a botanical that has been used in animal experiments to terminate a pregnancy. This has been attributed by several researchers to a strong Th1 cytokine response, particularly IFN-gamma and TNF (1). Increases in CD8 cells have been reported. Talwar reports that Neem has “inhibitory action on a wide spectrum of micro-organisms, including candida albicans, C tropicalis, gonorrhoeae, the multidrug-resistant Staphylococcus aureus and urinary tract Escherichia coli, Herpes simplex-2 and HIV-1.” (2)

Neem seed extract is reported to contain liminoids, some of which are toxic to certain cancer cell lines. (3). A 10 week study with Neem for its adverse effects showed a decrease in testosterone in wistar rats, who also had increases in white and red blood cells and lymphocyte counts without showing any cytotoxic effects. (4)

With one report that Neem has inhibitory action against HIV and promotes Th1 cytokines, this herb is worthy of consideration. Because it may reduce testosterone levels, the use of Ginseng with Neem would be synergistic as Ginseng increases testosterone levels. Neem is another treatment to promote Th1 cytokines that may also reduce HIV and the herpes viral loads. A Neem leaf powder in capsule form is available from “Natrol.”

I have no dosage recommendations except to suggest to follow the manufacturers recommendations. There are no studies I am aware of with Neem as a treatment for AIDS, CFIDS and candidiasis. Neem is plentiful and inexpensive. If you decide to use Neem, inform your physician and monitor your lab results as well as how you feel and let us know what you think of its effects, good or bad. When it comes to herbs like Neem with strong anti-microbial properties, I think it might be wise to pulse its use, that is, to use it just 3 days per week as the highest recommended dose. When we obtain reports of persons who use it continuously versus those who pulse it, we will be in a better position to recommend dosage levels as well as how to use it.

1. Talwar et al; Am J Reprod Immunol 1997 Jun;37(6):485-91

2. Talwar et al, Immunol Cell biol 1997 apr;75(2):190-2

3. Cohen E et al; Life Sci 1996;58(13):1075-81

4. Parshad O et al; West Indian Med J 1994 Sep;43(3):71-4.

Garlic - promotes Natural Killer cell function/Interluken 2 (IL-2) - kills HIV and Mycobacterium avium complex (MAC).

Tang Z et al reports that garlic can prevent cancer in experiments on Wistar rats. He states that this effect may be due to garlic’s ability to activate NK cells, the function of T-lymphocytes and the level of IL-2. (1) Tang determined these results by radioimmunoassay in an experiment on 32 Wistar rats in a controlled study. Previously, I reported in a past issue of this newsletter where 5000 mg (5 grams) of aged garlic extract daily restored Natural Killer cell function in a small group of HIV+ persons in 6 weeks. Raw garlic is far more powerful than aged garlic extract in directly killing viral, parasitic and fungal infections. If fact, there is no evidence that garlic capsules will directly kill any virus even though aged garlic has immunological value in activating NK function.

“At low concentrations, the drug (raw garlic) appears to have little toxicity, and its anti-HIV activity is 45 times more powerful than the drug dextran sulfate”. (2)

The article reports that the anti-HIV properties is found only in raw garlic. The substance in raw garlic that inactivates HIV is called “Ajoene.” Research done in Russia at Moscow State University indicates that ajeone found in raw garlic is a fusion inhibitor (3)

Research done at the Morehouse School of Medicine by Deshpande RG et al found that garlic inhibits MAC isolates form AIDS Patients (4)

Note: In persons with seriously damaged mucosal membranes, raw garlic can be irritating. It must be buffered by eating it with whole grain bread or crackers (i.e. rye crisp). If fresh parsley is added, it will help deodorize the garlic. Most readers report that 3 cloves of raw garlic daily has a powerful effect against fungal infections and especially when cold pressed olive oil is also used daily.

1. Hunan I Ko Ta Hsueh Pao 1997;22(3):246-8

2. Treat Update. 1998 May;10(3):1-2

3. Int Conf AIDS. 1992 Jul 19-24;8(3):39(abstract no PuA 6173)

4. J Antimicrob Chemother. 1993 Oct;32(4):623-6

Vinegar kills HIV

A study done in Glasgow on heroin users who combined heroin with vinegar prior to injection found both white and brown vinegar inactivated HIV. At a vinegar to water ratio of 1:3, they report “Both white and malt vinegar solutions inactivated cell-free HIV at concentrations 100 times the amount of virus needed to infect an assay system containing 10(5) CEM cells. These vinegar solutions also inactivated cell-associated HIV at a concentration of 10(4) infected cells/ml solution..” They concluded that vinegar “might have contributed towards keeping the seroprevalence of HIV among Glaswegian IDUs at a relatively low level of 6%.”

(1) 1. Goldberg DJ et al, Rucjill Hospital; Int Conf AIDS 1989 Jun 4-9;5:768(abstract no Th.D.P.55)

A Quadruple Natural Cocktail?

Question of the year: How many billions HIV viruses can you kill by eating a salad every day with 3 cloves of fresh sliced garlic plus 3 tablespoons of vinegar and 3 tablespoons of cold pressed olive oil with oregano added? Japanese researchers have found that oregano inactivates the HIV virus (1). Other research indicates that oregano kills a number of fungal and bacterial pathogens as well.

1. Katsuhiro et al; Biol Pharm Bull, vol 21, No 8; 1998, p 829-33

Lack of L-Glutathione induces Th2 responses in antigen presenting cells

The value of restoring intracellular Glutathione levels on antigen processing and presentation and activating CD8 CTL’s was reported in Positive Health News, Report No 16. Glutathione has a very role as an anti-oxidant and removes many toxins form inside the cells. New research indicates that a lack of intracellular glutathione leads antigen presenting cells to shift from a Th1 to a Th2 cytokine profile in response to infections.

Peterson JD et al reports:

“By using three different methods to deplete glutathione from T cell transgenic and conventional mice and studying in vivo and/or in vitro responses to three distinct antigens, we show that glutathione levels in antigen-presenting cells determine whether Th1 or Th2 response patterns predominate.” (1)

Substances that increase intracellular glutathione levels include alpha lipoic acid, N-acetyl cysteine, L-cysteine, selenium and L-glutamine.

1. Proc Natl Acad Sci USA 1998 Mar 17;95(6):3071-6

DHEA or AED promotes Th1 and decreases Th2 cytokines

Research done in Mexico by Hernandez-Pando R et al in mice exposed to B. Tuberculosis (TB) found that either DHEA (Dehydroepiandrosterone) or AED (androstendiol) had antiglucocorticoid effects and promoted Th1 cytokines. They reported that immunity to TB requires a Th1 cytokine profile. Their findings suggest that adrenal exhaustion may promote a cytokine shift from Th1 to Th2. (2) They report that AED was particularly protective, causing a “fall in bacterial counts and prolonged survival.”(2)

Research done by Inserra P et al at the University of Arizona found that in aged mice, the use of DHEA significantly increased Th1 cytokines, IL-2 and IFN-gamma and decreased Th2 cytokines - IL-6 and Il-10. (1) The abstract did not state the dosage used.

Editor’s note: Persons considering using DHEA should first have their physician check their DHEA levels. If they are low, you can take supplements to increase the level. suggested dose: Men: 25 to 50 mg daily. Females: 5 to 10 mg daily. I don’t think it is wise to take several hundred milligrams daily. It is even more unwise to place all your Th1 eggs in one basket. It is better to select several thing that support Th1 cytokines and to use each in moderation than to take an extreme amount of just one of these. Equally important is to strictly avoid all those substances that drive Th2 cytokines - the most common being vegetable oils (soy, corn, safflower, canola, sunflower) and sugar (glucose). The person who lives on canned soda, pastry and candy bars will undo many of the benefits he obtains from supplements that support Th1 cytokines.

1. Proc Soc Exp Biol Med 1998 May;218(1):76-82

2. Immunology 1998 Oct;95(2):234-41.

The Sun and Ultraviolet Light A (UVA)

UVA induces Th1 while UVB induces Th2

Sunlight has been blamed for skin cancer in many published studies. For years persons with HIV have been told to stay out of the sun on the assumption that it activates HIV. Controlled studies have contradicted these earlier beliefs and showed that sun tanning did not increase HIV viral loads. My position for years is that sunlight is healthy for most persons. It increases white blood cell counts, improves delayed type hypersensitivity (DTH), improves calcium absorption and stimulates deeper restful states of sleep.

Sunlight is a source of ultraviolet light, UVA primarily with some UVB and a small amount of UVC. Kondo S and Jimbow K report in the Journal of Cell Physiology (1) that UVA promotes Il-12 but not IL-10. They state:

“Considering that IL-12 promotes activation of Th1 cells and prevents activation of Th2 cells...our results suggest that UVA modulates skin immune function distinctively from UVB.”

Other researchers have pointed out the difference between UVA and UVB in terms of cytokine production. Skov L et al report that exposure of the skin to ultraviolet B (UVB) light causes immunosuppression which is relevant to the induction of skin cancer. They studied both UVA and UVB light on the skin and found their cytokine effects were opposite(2). They found UVB increased Il-10 significantly. They did not find this with UVA. With UVA, they found a decrease in TNF-alpha while they found a significant increase in TNF-alpha with UVB. Other researchers have found an increase in skin cancer in persons with psoriasis who undergo extensive UVB light treatments.

In my opinion, exposure to sunlight is safe and healthy if you eat a wholesome diet rich in antioxidants and one that does contain lots of immunosuppressive vegetable oils high in polyunsaturated linoleic acid. Persons who rarely eat fruits and vegetables and consume significant quantities of foods fried with liquid or hydrogenated vegetable oils should stay out of the sun. A small quantity of UVB in sun light could place at risk for skin cancer as their diet has already placed them at risk for cancer. If they don’t get skin cancer, they probably will some other type. With indoor sun tanning salons that exclusively use UVA light, I see not only no problem, but rather a lot health benefits.

1. J Cell Physiol 1998 Dec;177(3):492-8

2. Br J Dermatol 1998 Feb;138(2):216-20

Acupuncture increase NK activity and IFN-gamma

In studies on mice, electroacupuncture increased Natural Killer cell activity, although it did not increase the quantity of NK cells. Electroacupuncture caused the increase in NK activity by increasing beta-endorphin production and also increased IFN-gamma in the spleen. (1)

In a controlled study on 25 cancer patients and 20 controls by Wu B et al, acupuncture given for 30 minutes daily for 10 days increased IL-2 levels and NK cell activity. Pressure points used were ST36, LI11 and RN6. In the placebo group, NK cell activity and IL-2 levels remained below normal and unchanged. Wu concluded: “acupuncture therapy could enhance the cellular immune function of patients with malignant tumors ..providing a beneficial effect in anti-cancer treatment.” (2) Other published articles I’ve read confirm these findings.

For some time, I’ve heard from our readers reports of increases in CD4 counts with acupuncture. Persons with CFIDS, cancer and HIV would all benefit from acupuncture. Be sure to specifically ask for pressure points ST36, LI11 and RN6.

1. Yu Y et al; J Neuroimmunol 1998 Oct 1;90(2):176 -

2. Chung Kuo Chung Hsi I Chieh Ho Tsa Chih 1994 Sep;14(9):537-9 (Article in Chinese)

Vitamin E induces IL-2, NK function and IFN-gamma

Three different published studies have demonstrated that vitamin E supplementation in mice with AIDS (LP-BM5 retrovirus) increased IL-2, IFN-gamma and NK cell activity and decreased IL-4 and IL-6. Vitamin E also alleviated AIDS symptoms of splenomegaly and hypergammaglobulinemia. Vitamin E also reduced NF-kappa B which can cause injury to the liver by its stimulation of free radicals. In AIDS, NF-kappa B is excessive as is oxidative stress from free radicals. The same is true for cancer. Vitamin E also reduces the level of lipid peroxidation. (1,2,3,4). Research indicates that Vitamin E may benefit persons with impaired kidney function and sepsis. Its protective effects on the heart are already well established.

Suggested dose: 400 mg daily for adults. Best choices are alpha-tocopherol succinate or d alpha-tocopherol acetate - not mixed tocopherols.

1.Immunopharmacology 1995 Apr;29(3):225-33

2. Am J Vet Res 1995 Feb;56(2):179-94

3. J. Nutr 1994 Oct;124(10):2024-32

4. Clin Immunol Immunopathol 1994 Jul;72(1):70-5

Transfer Factor, Naltrexone, thymic factors and other Th1 promoters

Other substances that support Th1 cytokines include Transfer Factor, Naltrexone, NK911, IP6, Thy-Mate or thymic factors and DNCB. Licorice root, Dong Quai, silica and digestive enzymes reduce antibody production (i.e. Th2 humoral immunity).

Note: Licorice root can sometimes increase blood pressure. Start off with small doses and test its effects.

TH1 Summary

improves cell-mediated immunity

1. Omega-3 fatty acids (DHA & EPA) found in cold water fish, salmon, sardine and cod liver oil (improves DTH, lowers triglycerides, TNF and IL-6) - helps prevent heart disease and cancer. Flaxseed also contains some omega-3 fatty acids.

2. Monounsaturated fats found in olive and hazelnut oils (reduces TNF and increases IgA - supports mucosal immunity). Adult therapeutic dose: 4 tablespoons daily.

3. Vitamin A (increases transport of IGA - supports mucosal immunity) . Cod liver oil 2 tbsp daily.

4. L-Glutamine - support structure of mucus membranes, mucosal immunity and Glutathione levels

5. Silica - reduces IgG and improves NK function.

6. Digestive enzymes - improves digestion and assimilation of proteins and other nutrients, reduces circulating immune complexes that cause antibody and autoantibody formation. Protein digestive enzymes are found naturally in fresh ginger root, raw pineapple and kiwi fruit. (quality proteins support mucus membrane integrity). Additional factors that support digestion are:

a. Cayenne - 1 or 2 capsules taken before meals stimulates hunger and digestive enzymes. (support digestion of nutrients for mucosal membrane integrity)

b. Lemon juice or apple cider vinegar - taken with meals stimulates hydrochloric acid for protein digestion. (support digestion of nutrients for mucosal membrane integrity). Vinegar, any type, kills HIV and may other pathogens.

c. B vitamins - several kinds are needed to support hydrochloric acid production that is needed for protein digestion. B6 is very important for CMI.

d. Magnesium - needed by pancreas to produce pancreatin - used to digest proteins.

7. Friendly intestinal flora

a. Lactobacillus Plantarum and L casei - potent inducers of IL-12 and IFN-gamma. Supports mucosal immunity.

b. Bifidobacterium longum - increases IgA - supports mucosal immunity - reduces candida albicans - improves lactose tolerance.

c. Acidophilus - promotes resistance to colonization of candida albicans.

8. Ginseng (Red Korean or concentrated Siberian Ginseng extract) - increases IL-2, IFN-gamma and NK function.

9. Chlorella- broken cell wall - use liquid, granules or capsules only - not tablets with binders. Increases IL-12, GM-CSF and activates macrophages. Note: Spirulina and some other sea vegetables may have similar benefits - data lacking.

10. L-Thyroxine (T4) a thyroid hormone that is a potent inducer of IFN-gamma. Sources: Am J Physiol 1997 Oct;273(4 Pt 1):C125-32 and J Immunol 1998 Jul 15;161(2):843-9

11. Soil Based Organisms (SBO’s) (bacillus subtilis and lichenformis) - produce surfactin that inactivates lipid envelope viruses (HIV, CMV, herpes etc), kills mycoplasmas and many bacteria and candida albicans. By reducing candida albicans, SBO’s reduce Th2 cytokines.

12. Garlic - raw or aged extract - promotes NK function and IL-2. Raw garlic kills HIV, many kinds of fungus and bacteria including MAC.

13. L-Glutathione - promotes antigen presentation and stimulates CD8 CTL’s. An antioxidant, it neutralizes free radicals. Alpha Lipoic acid, selenium, NAC, cold processed whey proteins, certified raw milk and L-glutamine support increased Glutathione levels.

14. DHEA or AED (androstendiol). - increases IL-2, IFN-gamma and decreases IL-6 and 10.p 15. Acupuncture (points ST36, LI11 and RN6) increase IL-2, IFN-gamma and NK function.

16. UVA light - promotes IL-12.

17. Vitamin E - increases IL-2, NK function and IFN-gamma. Reduces NF-kappa B.

18. Transfer factor (antigen specific) - protein immuno-modulators extracted from colostrum from immunologically stimulated animals that promotes DTH and specific immunity to certain antigens (viruses etc.).

19. Colostrum - contains IgA - promotes mucosal immunity and immunity to specific antigens to which the animal was exposed.

20. Naltrexone - promotes NK function and resistance to candida albicans, thus reducing Th2 cytokines.

21. IP6 - found in brown rice and corn - promotes NK function.

22. NK911 - a transfer factor that stimulates NK function.

23. Lentinian and certain other mushrooms - promote Th1 cytokines and NK function.

24. Thy-Mate and thymic factors (Bio-Pro thymic Protein A) - increases IL-2 and T cell counts.

25. DNCB - promotes DTH and CD8 CTL activity

26. Licorice root and Dong Quai - reduces antibody production.p 27. Beta 1, 3 glucan - found in the common yeast and in oats and oat sprouts/rye sprouts - stimulates macrophage and neutrophil function. Note: may also spike IL-6 levels indicating a cross-regulatory role.

28. Noni -Tahitian - 2 tablespoons twice daily - promotes NK function and immunity against cancer.

29. Neem - promotes IFN-gamma and increases CD8s - also, a powerful antiviral, antifungal and antibacterial herb.

30. Gingko Biloba - reduces cortisol production that suppresses Th1 cytokines.

31. Exercise - aerobic - light and fun. Walking, gardening, dancing, sports. increases endorphin levels - improves NK function - removes toxins from body.

32. Water- Drink 8 to 12 glasses daily - removes toxins - reduces stress on adrenals, liver and kidneys.

33. Positive attitude and prayer. - ability to forgive, compassionate, willingness to help others. Long term goals, not just daily and a will to live. Reduces stress on the adrenal glands.

Note: This list is not complete. A further search of the scientific literature may result in further additions.

Th2 Summary Factors that induce Th2 cytokines and suppress cell-mediated immunity.

Note: items 1 and 2, processed vegetable oils and sugar are the most commonly used foods that depress cell mediated immunity in the American diet.

1. Processed heated vegetable oils high in trans-fatty acids and linoleic acid (safflower, soy, canola, corn and sunflower). Unless they are cold-processed or expeller pressed, they are already damaged goods by the time they reach grocery shelves and very immunosuppressive. They can also cause heart disease and cancer. Processed foods like french fries, potato chips, corn chips and pastry are usually load with these oils.

2. Glucose (white sugar) -candy bars, pastry, soda etc - suppresses function of all white blood cells, particularly macrophages.

3. Asbestos

4. Lead, mercury and other heavy metals

5. Pesticides, air and water pollutants

6. Progesterone

7. Prednisone

8. Morphine

9. Tobacco

10. Cortisol

11. Anal sex leading to discharge of semen

12. HIV, candida albicans, HCV, E coli and many other pathogens.

13. Stress (continuous) - emotional, financial, fear and worry

14. Thalidomide

15. UVB

16. Pregnancy - A Th2 cytokine predominant profile is needed. A strong Th1 cytokine profile including TNF can cause an abortion. Immune system rejects fetus.

17. Melatonin? conflicting research suggests that high levels induce Th2 cytokines while very small amounts induce Th1 cytokines.

18 Alcohol - Note: Studies on animals show that ethanol (alcohol) definitely suppresses Th1 cytokines and induces Th2. However, beer was not tested. In several anecdotal cases I have followed for several years, drinking beer alone, without hard liquor, has slowed down or even stopped HIV progression to AIDS. The factor in beer that might be doing this is hops, a herb that needs to be investigated to determine if it enhances Th1 and suppresses Th2 cytokines.

19. Streptococcus Thermophilis - a bacteria often used in making commercial yogurt, even yogurt that has acidophilus added may also have S Thermophilis.

20. Candidiasis (systemic candida albican infection) - stimulates Th2 cytokines.

21. Circulating immune complexes (CIC’s) - caused by a combination of leaky gut syndrome and poor digestion of proteins due to a lack or HCL and digestive enzymes. Also caused by semen ejaculation during anal sex in persons with impaired mucosal immunity.

22. Sedentary life - lack of exercise - leads to a build up of toxins that weakens CMI.

23. Water - lack of thirst - leaves to toxic overload and depressed CMI.

24. Negative attitudes - suppressed anger/rage - inability to forgive, resentful, hold grudges - stresses the adrenal glands and increases cortisol levels leading to adrenal exhaustion.

25. Low body temperature

26. Acid saliva pH

27 Chronic insomnia - inability to get a good night’s sleep - inability to dream.

28. Weight lifting - muscle tearing increases cortisol levels.

29. Steroids - for muscle gain - some are very hard on the liver and adrenals - suppress Th1 cytokines.

Note: This list is not complete. A further search of the scientific literature may result in further additions.

I want to thank John Sexton for the files he sent me which helped immeasurably in the preparation of this article. Due to limited space, persons wanting scientific data on numbers 2 through 18 can contact John Sexton at 808-965-7247 and he can e-mail them to you. His e-mail is 2150john@gte.net or you can do your own search on medline at http://igm.nlm.nih.gov/

Note to readers: You may photocopy the Th1 and Th2 Summary lists and post them for your reference needs.

Pulsed Protocols

Some substances induce both Th1 and Th2 cytokines including echinacea, astragalus and beta 1,3 glucan. These substances are best used in a pulsed protocol, 1 to 3 days per week and not continuously. Excessive intake of zinc is immunosuppressive and a lack of zinc is immunosuppressive. A safe dose range for adults is 25 to 50 mg daily.

Vitamin C activates Natural Killer cell function if used in a high dose (5000 to 10,000 mg) 3 days per week only. When used every daily, the body creates an enzyme to inactivate the Vitamin C rendering it ineffective.

Certain herbs when used every day become ineffective after a few weeks as the body produces either antibodies or enzymes to inactivate them. The best way to restore their effectiveness is to stop using them for one week, then to use them for no more than 3 consecutive days per week (i.e. 3 days use, then 4 days do not use). This is why I suggest that certain products be pulsed while others can be used daily. Almost any substance used as a medicine or drug can fail if the dose is too low to be therapeutic or too high for too long so as to induce an antibody response or enzyme response against the substance. When the latter happens, a pulsed or intermittent use of the substance will usually restore its effectiveness.

Hepatitis C (HCV) update

Transfer Factor, Lactoferrin and Amantadine

Standard treatments for HCV usually includes the use of alpha interferon and sometimes Ribovarin. Although some persons respond well to these prescribed drugs, there are reports of serious side effects and treatment failures.

In the last issue of this newsletter, I reported on Transfer Factor ABC (Chisolm Biological Labs) as a treatment for hepatitis A, B and C. Two readers report that after using one capsule daily for three months, their HCV viral load declined 80% and 90% respectively. No adverse effects have been reported.

Lactoferrin: Researchers Ikeda M et al from the Nat’l Cancer Instit in Japan report that bovine lactoferrin directly bind to the HCV and effectively prevented hepatitis C in cultured human hepatocytes cell lines. They report on experiments that show that Lactoferrin directly binds to HCV and not to the infected cells(1). Other research has confirmed that lactoferrin binds to HCV and a number of other viruses. Lactoferrin is also a natural component of human mother’s milk and is also effective against HCV. Lactoferrin is available as an over the counter dietary supplement and is available through companies like Jarrow Formulas. Adult dosage levels are usually 1000 to 1500 mg or more daily. No adverse effects have been reported.

1. Biochem Biophys Res Commun 1998 Apr 17;245(2):549-553

Amantadine. John Sexton, who sent me 60 files on Th1 and Th2 has been using lactoferrin and a low-cost prescribed drug called “Amantadine” for the treatment of HCV. The dose he said he is using is 100 mg twice a day. A study with Amantadine done at the Dept of Medicine at Milton S Hershey Medical Ctr by JP Smith reports that in 22 patients with chronic hepatitis C given 100 mg twice a day for an average of 32 months(1). The patients had previously failed interferon-alpha 2b therapy. He reports 64% of the patients had decreases in ALT values with 27% having normalization of ALT values and a loss of HCV RNA as measured by PCR. No side effects were reported.

1. Dig Dis Sci 1997 Aug;42(8):1681-7

HIV Progression to AIDS blocked for 15 years

An Interview with Michael Zielinski

1/27/99. A long term survivor who claims to have led a promiscuous life and practiced unsafe sex for several years in the late 1970’s and early 80’s and claims even to have had sex with Gaton Dugas, also known as “patient zero” and referred to in the book by Randy Schultz “And The Band Played On” tells Keep Hope Alive how he stopped HIV progression to AIDS for the past 15 years. He first became sick in 1983 with stomach aches and fevers and began self treatment with Chinese herbs and acupuncture.

Mark: What was in the Chinese herbs you took in 1984?

Mike: Don’t know. I just took them and know they helped me.

Mark: What else did you do?

Mike: I started eating 3 or 4 cloves of raw garlic daily and have been doing this for the past 15 years. I use at least the juice of one lemon daily and 4 or 5 tablespoons of Extra Virgin Olive oil. I’ve been doing this also for the past 15 years.

Mark: What was the lowest point in your CD4 cell counts?

Mike: For the past 9 years since I first started testing the T cells, they have consistently stayed in the 350 to 400 range.

Mark: Did you ever use any prescription anti-viral drugs during this time?

Mike: For the first 13 years, no. My viral load got to a high of 50,000 at the end of 1996 and I started on D4T and 3TC and continued with my diet and supplements. I never used any protease inhibitors, My viral load went non-detectable and has stayed there for the past 2 years and 3 months.

Mark: Was there anything else you took for the past 15 years that you used consistently?

Mike: I used 250 mg of BHT daily and digestive enzymes and HCL with pepsin. I also used 2000 mg daily of buffered vitamin C and one milk thistle capsule and any brand of acidophilis plus 10,000 i.u of beta carotene daily. I also took 600 mg daily of NAC for the past 15 years. For the past 3 years I also took gingko biloba daily. Besides that I run 2 or 3 miles every other day. For as long as I have been infected, I also take a multiple vitamin and mineral formula - have used different brands.

Mark: Did you use any oil besides olive oil?

Mike: Yes, I used almond oil when I make pancakes.

Mark: Almond oil is also high in monounsaturated fats. Are you saying you did not use soybean oil, corn oil, safflower, peanut and sunflower oil?

Mike: That is correct.

Mark: Thank you for the interview. Today Mike’s viral load is non-detectable and his CD4 counts is at 376.

Other factors in Mike’s Protocol

Mike told me he occasionally uses a “Parasite Cleanse” formula by “Michaels”, Essiac formula, cat’s claw, ginger root, eyebright, bilberry, blue green algae, zinc picolinate, saw palmetto, Pau d’arco, echinacea, castor oil packs, elderberry and glucosamine sulfate and colostrum. He says colostrum makes him feel great. He reports he started doing garlic and chlorophyll enemas in 1993. Mike protocol looks like a page taken out of this newsletter on factors that support Th1 cytokines, except he started 15 yrs ago. Mike can be reached at 214-351-5594 for more information.

6 common factors link Michael Z’s protocol to Marc Correa’s

There are several similarities between Michael Zielinski’s protocol and that of the even more successful Marc Correa protocol. Correa’s results are the most impressive of any ever recorded in nutritional and immune-based therapies. He started with a viral load of over 200,000 and at one point a CD4 count of zero. His turnaround is nothing short of miraculous. Today, his CD4 count is 800 and viral load is non detectable and has been since last spring. He accomplished this without taking any prescribed antivirals. The complexity of his protocol is not an easy act to follow and initially included 5 enemas weekly. Among the many common factors that linked both protocols are Extra Virgin Olive Oil - 4 tablespoons daily, 3 to 5 cloves of raw garlic daily and the use of fresh lemon juice daily. Correa uses 4 lemons daily while Michael Z used an average of 1 lemon. Both Correa and Michael Z exercise regularly. Exercise helps remove toxins from cells and expel them through the kidneys, perspiration and respiration. There is another striking similarity - it is the antioxidant - BHT.

Marc Correa used about 1000 mg of BHT (Twinlabs) daily for several months and it helped put the CMV in remission and saved his eyesight. Michael Z has been using 250 mg daily for the past 15 years. CMV shares over 60% or its genetic properties with HHV-6, the variant A strain. Foscarnet and Ganciclovir used to treat CMV Retinitis are also known to inactivate HHV-6A. Could this be true of BHT as well?

One person with CFIDS and who had HHV-6A confirmed by lab tests was using 500 mg daily of BHT. Then she decided to stop using BHT thinking it was not doing much good. Immediately, her symptoms got worse. She told me “I didn’t appreciate the value of BHT until I stopped using it....my symptoms got worse in a few days.” Just possibly BHT is inactivating HHV-6A. BHT is well known to inactivate other types of herpes virus including genital herpes.

In my book, I have long advocated the belief that HHV-6A is a major co-factor in HIV progression to AIDS. Could the mere 250 mg of BHT that Michael Z took daily for the past 15 years help stop HIV progression for the first 13 years of his infection. His CD4 count never fell below 300. Michael Z just swallowed one capsule daily with meals while Marc Correa predissolved the BHT in olive oil, which is a more effective method for assimilation. Perhaps, we should take a closer look at BHT. The National Institute of Health still needs to address and define the role of HHV-6A in AIDS and CFIDS.

Both Correa and Michael Z also took multiple vitamin and mineral formulas. Marc Correa used Complete Thymic Formula or Thy-Mate and Michael Z used various brands throughout the years. These 6 factors (exercise, olive oil, garlic, lemons, BHT and vitamin/minerals) link these two very successful protocols.

Suggestion for using BHT. Dissolve 8 capsules of Twinlabs BHT (250 mg each) in one pint of olive oil or 16 capsules to a quart. Each 4 tablespoons of this olive oil will give you 250 mg of BHT. Based on the Correa and Zielinski protocols, 4 tablespoons daily of cold pressed or Extra Virgin Olive oil is the suggested therapeutic dose. Michael Z used most of his daily olive oil in pasta dishes. Gluten-free pasta made from brown rice or quinoa are available in health food stores. Neither person was a vegetarian. Both ate meats cooked at low temperatures. Correa cooked his meat dishes in a Crock Pot - at about 190§F. Cooking meats at below the boiling temperature of water gives you the most easily digestible proteins possible in your home.

Update: I spoke to Marc Correa on Feb. 1st. He now takes L-glutamine - 1 tablespoon twice a day (Jarrow Formulas). His doctor told him it helps with L-glutathione synthesis and nucleotide synthesis in the gut. He also uses small amounts of licorice root to support the adrenal glands and has added alpha lipoic acid. He feels so good he rarely does enemas any more and does DNCB only once every 2 or 3 months as the reactions to the weakest dose are so strong.

Note: His phone number is available through Keep Hope Alive. He asked me not to publish it but to screen requests so he is not overwhelmed with phone calls.

Jerry Cunningham

March 6, 1999. I talked with Jerry Cunningham today. His health turned around (viral load dropped to non-detectable levels and CD4’s increased) rapidly when he went on a triple combo - Viracept (protease inhibitor) along with D4T and 3TC. However, the combo left him with chronic diarrhea which did not resolve after 8 months. About one month ago, after talking with Marc Correa, he started using the whole lemon/olive oil drink twice a day (2 lemons and 2 tablespoons of olive oil per drink) plus he ate 5 cloves of raw garlic daily. After one week, the diarrhea stopped and has not returned. Jerry can be reached at 208-378-8145 for more information.

Questions and Answers.

Q: Why is it so difficult to shift the cytokine profile form Th2 to Th1 and restore cell-mediated immunity?

A: Certain infections like candidiasis and other infections promote the production of Th2 cytokines. Toxicity, adrenal exhaustion and impaired thyroid function also have major roles in the shift to Th2. Toxicity affects all major organs. Until this issue of Positive Health News you are now holding in your hands, we did not know all the factors that can shift the cytokine profile in favor of cell-mediated immunity. Now we have a road map before us - 33 factors that favor Th1 and 29 that support Th2. It is a good idea to look at the Th2 list first and eliminate as many negatives as possible.

Detoxify by drinking lots of clean filtered water, use enemas regularly, do light aerobic exercise to the point of sweating and drink raw vegetable juices. The Summary list of factors that support Th1 or induce Th2 is priceless but it not complete. There is more information in these areas in published scientific literature that has yet to be retrieved. At least now, we have a foundation upon which to build.

For some time, persons doing a few things to support Th1 cytokines were undoing the benefits by doing a lot of wrong things that stimulate Th2, the most common mistakes are high temperature processed foods containing trans-fatty acids from vegetable oils and sugar. This combination is so immunosuppressive that it can undo the benefits of a couple hundred dollars worth of good dietary supplements every month. All fried foods and foods cooked above 300§F should be placed on a list of suspected trouble makers.

Q: What is the most effective way to treat advanced cases of HIV infection or cancer?

A: “Hybrid protocols” or as Bob Lederer of Poz magazine wrote recently “integration” of prescribed drugs with nutrition and immune-based therapies. For advanced HIV infection where symptoms are serious, you may need both prescription and non-prescription anti-virals, anti fungals and antibiotics for bacterial infections plus nutritional and immune based therapies.

For HIV, readers have had good results with D4T and 3TC. Other combinations are Viramune, D4T and 3TC, Viracept with D4T and 3TC or Sustiva with D4T and 3TC.

For cancer, low-dose chemotherapy along with immune-based therapies has produced good results. Other persons have successfully combined venus fly-trap extract or Essiac herbal tea with Naltrexone, NK911 or IP6. The idea is to combine something that kills the cancer directly but has low toxicity with immune modulators that activate NK and LAK activity. Neupogen (prescription drug) by Amgen helps build the white blood cells. In anecdotal reports, some herb like bloodroot are toxic and should not be used internally although they work topically for skin cancers. One local person with leukemia has stabilized his condition by taking 2 tablespoons of Tahitian Noni daily for the past 6 months. Everyone with cancer should be given Naltrexone, 3 mg, once a day before bedtime. Naltrexone (a prescription drug) could save thousands of lives, but it costs too little and generates too small a profit to be seriously promoted in our market-driven economy. Naltrexone activates Natural Killer cells, a type of white blood cell that kills cancer cells.

Q: What over-the-counter antivirals for HIV have you been most impressed with over the past few years?

A: Olive Leaf Extract by East Park Research has for most persons been effective as an anti-viral agent for HIV. Suggested dose is one capsule 3 times a day or 2 every 12 hours. Other brands have far less oleuropein that the 38% found in the East Park brand. Some brands use only olive leaf powder with 5% oleuropein in it.

A good combo would be Olive Leaf extract with D4T and 3TC plus vegetarian enzymes. I have followed 6 cases where 2 or 3 capsules of vegetarian enzymes with each meal either lowered HIV viral loads or stabilized them and often with increases in CD4 counts.

Recently, Bob Mitchell tried the following protocols for 32 days and decreased his viral load from 340,000 to 41,000. He used 6 capsules daily of East Park Research olive leaf extract along with 2 St John’ Wort (Jarrow) capsules twice a day, 3 capsules of Curcumin (Jarrow) twice a day, 3 Source Naturals Essential Enzymes 3 times a day plus 4 drops of essential oil of Nutmeg massaged into his abdomen twice a day.

Since his last test Feb. 8th, he has added daily enemas of garlic and vinegar and water, 4 tablespoons of cold pressed olive oil and 2 tablespoons of Cod Liver Oil for DHA/EPA. He reports he is feeling better. He can be reached at 305-595-8092.

Editor’s note: I would rotate Curcumin after 30 days with Lemon Balm extract - 40 drops 3X for 30 days then with Oregamax, a wild crafted oregano for the next 30 days before going back to the Curcumin. Sulfated polysaccharides, glucosamine and chondroitin sulfate have reduced viral load for some persons, but not for others. If they work for you, use them. Dextran Sulfate is reported in several sources to inactivate HIV, but I don’t know where you can obtain it or how much to use. Another source of sulfur, MSM, has never been tested for its anti-viral effects, but should be. Sulfur based compounds are promising anti-viral treatments and more research funding is needed. Sulfa drugs (sulfur based) have been long known to be effective against many kinds of infections.

Q: What is the most effective product you have heard of to kill stubborn candidiasis and fungal infections?

A: Other than prescription drugs like Diflucan, it is Oregamax (Northern Spice and Herb Co). Several persons with CFIDS or candidisasis have told this is the most effective product they have found for Candida Albicans. Some persons have taken as much as 3 capsules three times a day. It is important to supplement with Biotin to keep the yeast in its single cell state where it is noninvasive. Japanese researchers have found that oregano also kills the HIV virus. The wild crafted oregano is the most potent product on the market. One lady told me she used oil of oregano (Northern Spice) in enema water and had excellent results. However, unless you turn on those Th1 cytokines, the candida will be back with the first sweet roll you eat.

Q: Have you had any reports on the use of enemas with garlic and vinegar?

A: Yes, today, I talked with a lady from South Carolina who had to quit a drug cocktail because of severe neuropathy. Her husband found our internet website and the February message on enemas and HIV infection in the intestines. She started on the enemas about 3 weeks ago for 5 days a week and rotated to chlorophyll enemas the following week. From the very first enema, she said she felt immediately much better. She said that today, this is the best she has felt in several years. She had blood drawn a few days ago and will know soon what effect these enemas have had on her HIV viral load. In Australia, a lady with CFIDS for 10 years started on daily enemas with garlic, vinegar and chlorophyll and reports she is noticeably improved.

Thanks to thousands of people who have requested the Pieta Prayer book and even more thanks to those who actually take the time to recite these prayers. I believe there is a real Divine intervention experience of which we are all part and for that we should all be thankful and appreciative. There is a veil of time and space that separates the physical reality around us from the spiritual dimension while communications pass to the other side with our thoughts and prayers. By Our Creator’s design, the spiritual realm will not likely ever be the object of scientific study. While few of us are privileged to see God and spiritual beings, none of us have seen the wind, but we can see and feel its effects, and thereby know of its existence.

Excerpts from the last 4 issues of Progressive Health News (Nov 1, 1998 through Feb 1, 1999)

HIV primarily infects and destroys CD4 cells in the intestines.

Mark Konlee - Feb. 1, 1999.

Michael J. Fox starred in the Hollywood movie “Back to the Future” while I now will step back in time to an article I wrote in the 4th edition of the “AIDS Control Diet” book on October 20, 1992, titled “A THEORY ON HIV PROGRESSION.” This theory is resurrected today as it is very relevant in light of the bombshell articles by Andrew Korotzer, Ph.D. in the current issue of Searchlight magazine called “The Gut Reaction to HIV” and “Whole Body Viral Burden” - hence Forward to the Past.

In my article written in 1992, I hypothesized the following: “Gastrointestinal tract inflammation caused by immune system attack on HIV....As the (HIV) virus grows in the intestinal tract, it passes through the intestinal wall and into the blood stream.” I also reported on the novel theory of Dr. Gerhard Orth of Germany that AIDS is caused by the destruction of intestinal mucus membranes by fungal infections. While I don’t agree with Dr. Orth’s theory that fungal infections alone of the intestines are the primary cause of AIDS, they are most likely a co-factor. I did find it plausible that damage to the mucus membranes could contribute to AIDS and I raised this interesting question: “As a spin-off to Dr. Orth’s theories, could the HIV virus be destroying the mucus membranes of the gastrointestinal tract?”

Today, there is no evidence that HIV actually destroys the mucus membranes of the GI tract, but rather, scientific research indicates that HIV uses the mucus membranes as fertile soil to replicate, infect and destroy CD4 helper cells that are adjacent in the lamina propria of the intestines.

The research of Korotzer, Fantini J, Yahi N and many others demonstrate that HIV can infect the mucosal membranes of the colon (large intestines) not only as a primary route to transmit HIV infection, but actually replicate in the mucosal membranes where the virus passes through the epithelium into the lamina propria where it comes in contact with activated CD4 helper T cells that are vulnerable to infection.

While it is well known that unprotected anal sex in gay men is the primary route for spreading the AIDS virus, it has been long assumed that this source of entry for HIV in the rectum is important only as it leads to HIV/AIDS as a blood disease.

WRONG! The latest information gleaned from several sources indicates that for the loss of CD4 helper cells, a hallmark for HIV progression to AIDS, there must be active and ongoing replication of HIV in the mucus membranes of the gastrointestinal (G.I.) tract.

It is well established that SIV is to monkeys what HIV is to humans as an immunodeficiency virus. An article published by Veazey RS et al in Science, April 17, 1998, titled “Gastrointestinal tract as a major cite of CD4 depletion and viral replication in SIV infection.” (1) reports the following:

“Human and simian immunodeficiency virus (HIV and SIV) replicate optimally in activated memory CD4+ T cell, a cell type that is abundant in the intestine. SIV infection of rhesus monkey resulted in profound and selective depletion of CD4+ T cells in the intestine within days of infection, before any such changes in peripheral lymphoid tissues. The loss of CD4+ T cells in the intestine occurred coincident with productive infection of large numbers of mononuclear cells at this site. The intestine appears to be a major target of SIV replication and the major site of CD4+ T cell loss in early SIV infection.”

1. Veazey et al; Science. 1998 Apr 17;280(5362):427-31.

Korotzer reports on research that demonstrates that even when SIV is injected into monkeys, within days, it has actively infected the intestines. While SIV initially entered the host via the blood, it preferentially replicates in the intestines. What is especially significant is that in monkeys, CD4 T cell depletion due to SIV infection in the intestines precedes SIV infection of the lymphoid tissues (lymph nodes). It is also significant that there are more complaints in persons HIV+ about the gastrointestinal tract at all stages of AIDS than any other single area of the human body.

This raises three important questions.

1. Should we not be targeting the intestines with anti-viral treatments for HIV?

2. Should we not be focusing on treatments to restore mucosal immunity to stop the replication of HIV in the membranes of the intestines?

3. What are the treatment options most promising to attain both objectives?

THE “AIDS RESEARCH ALLIANCE” BOMBSHELL

86% of all HIV in your body replicates primarily in the mucus membranes of the gut and in the lymph nodes. 40 to 60% of all the immune cells in your body are in your gut.

In the winter 98/99 edition of Searchlight, the official publication of AIDS RESEARCH ALLIANCE of West Hollywood, CA, is an article by Andrew Korotzer, Ph.D. titled “The gut reaction to HIV.” Korotzer cites research in 3 major scientific publications (1,2,3) including “Nature’s Medicine,” “AIDS” and the “Proceedings of the National Academy of Sciences” that indicates that about 14% of HIV replication occurs in the blood while 86% occurs the mucosal membranes of the intestinal tract and in the lymph nodes. IN OTHER WORDS, AIDS IS NOT PRIMARILY A DISEASE OF THE BLOOD, BUT RATHER OF THE GUT AND THE LYMPH NODES! These findings have profound implications in designing future treatments for HIV by focusing anti-viral therapy on the areas where the highest concentrations of HIV is found - the intestinal tract and the lymphatic system.

1. Dianzani F et al. Nature Medicine (1996) 2:832

2. Fackler O.T. et al. AIDS (1998) 12:139-146

3. Pantaleo G. et al. Nature (1993) 362:355-358

4. Wong J.K. et al. Proc. Natl. Acad Sci USA (1997) 94:12574-12579.

Korotzer states:

“Mucosal membranes in the rectum and the urogenital tracts serve as entry points for sexually transmitted HIV; therefore, viral dynamics in these tissues are particularly relevant to the etiology of HIV/IDS. This holds true even for lymphoid tissue in mucosal membranes when the initial infection occurs elsewhere. SIV, a virus related to HIV that infects monkeys, will become established in GALT (Gut-Associated Lymphoid Tissue) within days of infection - even when the virus was inoculated intravenously. This highlights the special vulnerability of GALT to infection with HIV.”

Korotzer cites 3 factors that makes the mucosal membranes of the gut vulnerable to HIV infection. They are 1. the presence of a large population of activated CD4 helper cells 2. mucosal membranes in the gut transport digested food material into the body for evaluation by immune cells and 3. surface molecules that HIV uses as a point to gain entry into the cells (i.e. CD4s) are highly expressed in the gut making them vulnerable to infection.

Other factors that Stress the Immune Response in Digestion

CD4 and many other types of white blood cells are in the intestines to inspect and determine the safety of the byproducts of digestion. Proteins and other nutrients that are not properly digested are attacked by the immune system when they get past the mucus membranes. Hence the importance of eating foods in a way to assist in their complete digestion, that is, eating slowly and mixing lot of saliva with each mouthful. The use of cultured, fermented, raw or living foods high in natural digestive enzymes or the taking of supplemental enzymes will assist in the digestion process. When you combine poor digestion with leaky gut syndrome caused by Candida Albican overgrowth, you have a combination that causes serious stress on the immune system and weakens the capacity of the immune system to deal effectively with infectious organisms elsewhere in the body.

This stress is further exacerbated when the mucus membranes are weak and there is a failure of mucosal immunity (i.e.. lack of IgA and CD8 cytotoxic lymphocytes in the mucosal membranes). These factors that can stress the immune response occur not only in HIV infection, but in HHV-6A, CFIDS, Candidiasis, Hepatitis, GWS, cancer and in persons with allergies and multiple chemical sensitivities.

LOWER CD4 COUNTS IN THE BLOOD LINKED TO HIV INFECTION OF THE MUCOSAL MEMBRANES OF THE GUT.

While it is well known that HIV preferentially infects activated CD4 cells, Korotzer reports that because CD4 cells in the lamina propria of the gut are highly activated, they present surface molecules that make them very susceptible to infection by HIV. The lamina propria are under a single layer of mucus membrane called epithelial cells. Korotzer reports that HIV clings to a type of cell in the mucus membranes called “M” cells and the M cells are thought to present antigen to the CD4 helper cells in the lamina propria. He states:

“M cells are the cell type within the epithelium thought to be responsible for the transport and presentation of antigen to lymphoid cells within. Lymphocytes migrate towards the M cells to become educated about the material the gut is being exposed to. Many of these lymphocytes are the activated helper T cells that are particularly vulnerable to infection with HIV. HIV can adhere to the surface of M cells, and it is possible that T cells inside the gut mucosa become infected when they migrate out to meet M cells.”

Korotzer states:

“there is a selective loss of CD4+ helper T cells within the lamina propria, and there are indications that helper T cells are depleted in the lamina propria before there is loss detected in the blood.”

Korotzer states that Dr. Peter Anton and associates at UCLA reported at the recent XII AIDS Conference in Geneva that using endoscopic rectal biopsies that chemokine receptors of T cells in the gastrointestinal tract made them particularly vulnerable to HIV infection. In a second article published in the same issue of Searchlight(1) called “Whole-body viral burden” Korotzer points to research that indicates that HIV replication activity in the gut is greater than in the lymph nodes or any other tissues in the body. In his article on “Whole-body viral burden” Korotzer states that “blood/plasma levels of HIV are just the tip of the iceberg.” He is collaborating with Dr. Peter Anton of UCLA to develop techniques to quantify the amount of virus lurking within tissue compartments of the body.

Korotzer raises two questions in conclusion to his article on “Whole-body viral burden.’ They are 1. Does HIV viral replication in the gut have an impact on the course of HIV disease? and 2. Does the high rate of HIV replication in the gut contribute to drug resistant strains of HIV?

1. Searchlight, 621A North San Vicente Blvd, West Hollywood, CA 90069 Winter 98/99 issue. Ph No 310-358-2423. Subscriptions available with a $35 donation.

More Scientific Research on HIV in the Gut and Lymphoid tissues.

1. “HIV-1 p24 but not proviral load is increased in the intestinal mucosa compared with the peripheral blood in HIV-infected patients.” Fackler OT et al. AIDS. 1998 Jan 22;12(2):139-46. Fackler states: “The high viral antigen load in the intestine therefore indicates that mucosal HIV production is upregulated at the transcriptional and/or translational level. The intestinal mucosa is a major reservoir for HIV in HIV-infected patients.”

2. “Tumor necrosis factor-alpha (TNF-alpha) stimulates bi-directional production of HIV-1 in polarized human colon epithelial cells,” Yahi N et al, Int Conf AIDS. 1993 abstract no PO-A13-0220) Yahi states: “TNF-alpha is a potent stimulator of HIV-1 replication in chronically infected differentiated HT29 (gastrointestinal epithelial) cells...”

3. “Perturbations of glucose metabolism associated with HIV infection in human intestinal epithelial cells.” Lutz et al, AIDS. Feb;11(2):147-55 Lutz states:

“HIV-1 infection results in a disturbance of glycolytic and oxidative activities in human intestinal epithelial cells.”

4. “Intracellular calcium release induced by HIV-1 surface envelope glycoprotein in human intestinal epithelial cells: a putative mechanism for HIV-1 enteropathy,” by Dayanithi G et al Cell Calcium. 1995 Jul.18(1):9-18

Dayanithi states: “HIV-1 may directly alter ion secretion in the intestine and thus be the causative agent of the watery diarrhea associated with HIV-1 infection.”

5. “Physical contact with lymphocytes is required for reactivation of dormant HIV-1 in colonic epithelial cells,” Faure E et al, Virus Res. 1994 Oct; 34(1):1-13

Faure et al state: “observations provide a putative molecular mechanism for transmission of HIV-1 from mucosal epithelial cells to lymphocytes.”

6. “Replication and apical budding of HIV-1 in mucus-secreting colonic epithelial cells,” Yahi N et al. J. Acquir Immune Defic Syndr. 1992 Oct 2(10):993-1000. Yahi et al state: “data suggest that HIV-infected goblet cells in the colonic mucosa may produce the virus in the colorectal lumen; this could explain the route of transmission of HIV in the case of anal intercourse.”

7. “Selected HIV replicates preferentially through the basolateral surface of differentiated human colon epithelial cells,” Fantini J et al. Virology. 1991 Dec; 185(2):904-7

Fantini et al state: “data suggests that epithelial cells of the colon productively infected with HIV are able to produce the virus through both sides of the epithelium but mainly through the serosal side.”

Other Infections of the Intestinal Tract

The large intestines (Colon) is a nest for the growth and replication of many pathogens that contribute to immune dysfunction and many symptoms. Published scientific research demonstrates that the gut can be heavily infected with HIV, parasites, candida albicans, CMV, tuberculosis, hepatitis viruses, cytomegalovirus and mycobacterium avium complex (MAC/MAI) to name a few.

TREATMENTS THAT EFFECTIVELY TARGET HIV INFECTION IN THE GUT COULD LEAD TO INCREASES IN T CELL COUNTS AND DECLINES IN PLASMA VIRAL LOADS.

Korotzer did not get into the issue of targeting treatment for HIV infection in the mucosal membranes of the large intestines, but I will. As readers know by now, I am not shy about giving out treatment advice. Several years ago I espoused the theory that AIDS progression was caused by massive replication of HIV in the colon that was absorbed into the blood. This was in the forerunner of my current book “How to Reverse Immune Dysfunction” called “AIDS Control Diet.” which had 6 editions before the title was renamed.

The reason for focusing on developing an effective treatment for HIV infection in the gut should be all but obvious. If the primary source of destruction of CD4 cells and HIV replication is occurring in the mucus membranes of the intestinal tract, then this is the area that we need to specifically target to reverse the course of HIV infection. The benefits of destroying billions of HIV viruses in the gut are that it will reduce the number of new CD4 cells infected with HIV and reduce the whole body viral burden including that in the lymph nodes. This will lead to a drop in plasma viral loads and an increase in CD4 counts in blood plasma.

In CFIDS, restoring mucosal integrity and immunity should substantially reduce symptoms and digestive disorders and improve the immune response against HHV-6A that causes neurological aberrations elsewhere.

TREATMENTS TO TARGET INFECTIONS IN THE MUCUS MEMBRANES OF THE COLON

MEDICINAL ENEMAS AND COLONICS.

Stanley Rebultan is now in his 10th year of daily bitter melon enemas as a monotherapy for HIV and is going strong. He has never had an opportunistic infection and his lab results show a completely normal functioning immune system. Is all the credit due to the bitter melon or was it the enemas themselves that have kept him alive and healthy? Enemas are a common link between Stanley Rebultan (the Bitter Melon man) and the highly effective Marc Correa protocol that has been reported in past editions of this newsletter. He went from a viral load of over 200,000 to non-detectable levels and has stayed there. Is it just a coincidence that he did enemas 5 times a week?

I know of several persons who have taken the same supplements Marc Correa has taken, but have not had the same spectacular results. Several times, I have been asked, “What is the secret of the Marc Correa protocol?” For the past year, my standard answer has been that it is probably the synergistic effect of the entire protocol. While this is true, I recently became convinced that the enemas were a key and critical component of that protocol, without which, the amazing results he has had would probably not have materialized.

12 TYPES OF ENEMAS

Twelve types of enemas are being suggested here.

1. Garlic and apple cider vinegar. (Place a pint of vinegar in a blender and add 8 cloves of raw garlic. Blend, strain and place vinegar back in the bottle. Use 1/4 cup for each enema treatment).

2. Aloe vera juice - 1 cup to a enema bag filled with water

3. Witch Hazel - 1 tbsp in a cup of water to make a tea.

4. Lemon Balm - 1 tbsp in a cup of water to make a tea.

5. Chlorophyll - 1/4 cup to an enema bag.

6. Ozone A water enema followed by ozone rectal insufflation.

7. Oxygen and water. Oxygen is bubbled into 2 quarts of water for about 5 minutes and then the water is used for an enema.

8. Urine retention enema. Add one cup of more of your first morning urine to the enema bag.

9. Coffee retention enemas.

10. Bitter melon enemas

11. Black walnut. (Add 1 or 2 teaspoons to the enema bag)

12. Charcoal. (1 or 2 teaspoons to the enema bag)

The Witch Hazel or Lemon Balm tea is then added to the enema bag and the balance is filled with lukewarm water. My initial suggestion is to rotate these enemas every 7 days. The anti viral enemas are Garlic and vinegar, Lemon Balm (for HIV and herpes), chlorophyll, charcoal, Bitter Melon (for HIV), urine, ozone rectal insufflation and the oxygen and water enema. Anti-parasitic enemas are garlic and black walnut. Anti-fungal enemas are garlic, vinegar and black walnut. Enemas to heal the mucus membranes are aloe vera, buttermilk, yogurt and witch hazel, slippery elm and marshmallow root. Enemas most effective in detoxifying the liver are coffee or ozone. Enema to implant good bacteria is yogurt or cultures like L Casei. Enema to absorb toxins, viruses and bacteria is charcoal.

Many of the enemas I listed here have never been tried. Many of the new ones are the result of my own research. The list I have written is not complete but it should be good for starters. As for safety, almost anything that is safe to place in your mouth is safe to place in your rectum with obvious exceptions (ie contaminated substances). You must have pure water that is not contaminated or you could pick up some new infection. City tap water in most location simply cannot be trusted and may contain cryptosporidium. Use distilled, RO or highly filtered water - down to 1 micron or less or to be sure, boil the water first for 5 minutes and let it cool before placing it in the enema bag. (Enema/douche kits are available at most drug stores.)

The bag should be mounted about 18 inches above the bath tub and the enema done lying in the tub face up. Self-sticking plastic hooks can be found at most hardware stores that will stick to ceramic or plastic on the wall. The colon is filled with solution and the shut off value is turned off when it feels full. The process of releasing the colon contents into the tub and refilling the colon occurs several times. If the enema is given after a bowel movement, there will be less cleanup time after the enema is done.

COLONICS AND ENEMAS FOR PERSONS WITH CFIDS, MCS or HEPATITIS

Enemas are best done once a day after a bowel movement and not after just eating. Persons with allergies and multiple chemical sensitivities (MCS) would especially benefit from aloe vera, urine, yogurt and charcoal enemas. Persons with CFIDS and HHV-6A infection would likely benefit from all the above enemas as would persons with HIV. Persons with hepatitis would benefit especially from charcoal and chlorophyll enemas but may benefit from others as well. Persons with candidiasis would benefit from the antifungal enemas and the ones to heal the mucus membranes. Persons with chronic diarrhea need to replenish fluid and electrolyte loss after doing an enema. Consider vegetable juice, fruit juices or blackstrap molasses.

THE MUCUS MEMBRANES - THE IMMUNE SYSTEMS FIRST LINE OF DEFENSE AGAINST VIRUSES ALSO PREVENTS ABSORPTION OF FOREIGN PROTEINS AND TOXINS THAT CAUSE ALLERGIES AND MULTIPLE CHEMICAL SENSITIVITIES

Healthy mucus membranes are a first line of defense against pathogens (i.e. HIV, HHV-6A, papilloma, herpes, hepatitis etc.) and foreign proteins from incompletely digested foods as well as toxins from putrefying bacteria and parasites. This underlying condition results from a 1. faulty digestive process and 2. leaky gut syndrome that is usually caused by fungal infections that puncture the mucosal membranes of the gastro-intestinal tract along with 3. parasites and putrefying bacteria that produce toxins and 4. the integrity of the mucus membranes themselves.

Published scientific research indicates that persons that have natural immunity to HIV, after multiple exposures through unprotected sex, have high levels of CD8 cytotoxic lymphocytes and Immunoglobulin type A (IgA) in the mucus membranes. The mucus membranes in the mouth, colon and vagina are a common route for the transmission of viruses like HIV, HHV-6A, papilloma virus, herpes and hepatitis. In December, 1998, researchers Belyakov et al at the National Cancer Institute report “We conclude that protection against GI mucosal challenge not only is CTL mediated but also requires local mucosal CTL at the site of challenge....We also found surprising persistence not only of memory CTL in the mucosa but also of protective immunity against mucosal viral challenge.” (1) The article also reported that IL-12 supported the CTL mucosal response. 1

. J. Clin Invest 1998 Dec 15;102(12):2072-2081.

ALOE VERA JUICE TO HEAL THE MUCUS MEMBRANES

Start with a gallon of aloe vera juice. Drink 1 cup 4 times a day for 4 days to use up the whole gallon. Drinking a quart of aloe vera juice daily for 4 days is for intermittent use only, repeated once every 3 to 6 months or as needed. After you finished your first gallon of aloe vera juice, feel the inside of your mouth and notice how much smoother it is. Then add daily supplements of Vitamin A, Silica, and L-glutamime (2000 mg to 20 grams or more daily).

LOCAL HYPERTHERMIA

Electric heating pad or Infrared light and sweating it out.

Whole body hyperthermia, where the whole body is overheated has been used for some time in the treatment of both AIDS and cancer with considerable success as well as some inherent dangers. The treatment is like an artificially induced fever therapy. Like fevers, when they get too high for too long, hyperthermia can be dangerous and cause brain, heart damage and in extreme cases, even death.

As the current research indicates that HIV activity is concentrated in the abdominal area, two ideas for a localized heat treatment that should be far safer than whole body hyperthermia are presented here.

1. One is the use of a electric heating pad over the abdomen area for one hour daily to directly kill viruses in the gut area. If the heating pad is turned on high, a flannel cloth should probably be placed between the heating pad and the skin to prevent burning the skin. A castor oil pack with heat pad treatment should accomplish the same purpose, although for heat treatment purposes alone, castor oil would not be necessary.

2. A second method is to place a infrared heat lamp above the abdomen area for a period of time each day. Infrared heat has good penetrating properties. As I haven’t worked with infrared light, I would advise anyone considering its use to get some professional advice on how close the bulb should be to the skin and for how long.

I would also advise anyone trying heat therapy to drink lots of clean water during and immediately after a heat treatment - 3 or 4 glasses of purified water to flush out toxins and destroyed pathogens. Induce sweating - yarrow tea and/or cayenne or hot salsa. Persons with low body temperature would most benefit from localized heat treatment in fighting almost any kind of infection.

North Pole Magnetic Field Therapy

Another treatment to reduce inflammation and viral activity in the intestines (or almost any other area of the body) is placing a strong north pole magnetic field over the abdomen for about 1 hour daily. Do this when the stomach is empty. A 4”x6”x«” permanent ceramic magnet will do or a magnet pad might work even better. More information can be found in the book “Biomagnetic Handbook,” by William Philpott MD. Call EnviroTech at 405-390-3499 for more information. Note: to induce deeper sleep, a 2” x 5” X«” magnet placed under the pillow works better than the 4”x6”x«” ceramic magnet which in my opinion is too strong. I speak from personal experience. North pole side of magnet should always be facing toward the body.

Note: a magnet should never be placed near the heart of someone with a pacemaker or the pacemaker will stop working. Magnets will also erase your credit cards and do a number on your CDs, cassette tapes and videos. To be safe, keep them at least 18 inches away from these items.

ARE YOU DREAMING?

IF NOT, TRY VITAMIN B-6

Your brain and the quality of sleep you get has a lot to do with endorphin and hormone production in your body. Without deep restful sleep, you will be constantly under stress and your immune system will likely be in the TH2 mode and your adrenals will be under stress. You may wake up feeling tired. Try 100 mg of Vitamin B-6 twice daily and it should help restore a deeper more restful sleep with normal dream patterns.

Elderberry Extract - 4 readers report increases in HIV viral load while stating in the same breath that they feel like a million dollars.

In an anomaly that I cannot explain, 4 readers of my last newsletter have reported a 3 to 4 fold increase in HIV viral load counts within 4 to 8 weeks after drinking home made elderberry extract according to a recipe published in my last newsletter. At the same time, all of them report feeling great, good energy, sleep deeply, have nice stools - floaters and look several years younger than they did after drinking the extract for about 2 weeks. Some used Glucosamine and Chondroitin Sulfate with the elderberry extract and some did not and the results are essentially all over the map.

One thing I can now conclude about Elderberry alone is that there is no consistent anti-HIV effect showing up in PCR nor are there consistent increases in HIV viral load. There is no information available on what effects elderberries are having on various cytokines. Does elderberry promote Th1 or Th2 or a little of both? Elderberries are high in anti-oxidants. However, if we consume too much for too long, does the body rebel against it in some way?

My latest thinking on elderberry is that it should not be used on a permanent daily ongoing basis, but could be safely used for 2 weeks on, then take 2 weeks off or use it only 2 or 3 days a week. In HIV, I see its primary benefits, not as an antiviral for HIV, but its metabolic and anti-oxidant benefits. It promotes deep restful sleep, large diameter floating stools, good energy, well being and healthy smooth skin.

ELDERBERRY for ALZHEIMER’S

Anti-Aging effects reported

Les and Susan O’Neill from Cabarita Bch, Australia, report that Susan’s mother, who has been diagnosed with Alzheimer’s, has had a complete remission of all her symptoms (loss of short term memory, interrupted speech, thought processes, etc.) by drinking 1/3 cup of elderberry extract twice daily for 3 weeks. Susan said her mother use to phone her 5 or 6 times a day and each time she called, she forgot that she had already talked to Susan earlier in the day. Now, she phones only once a day and they have a nice rational conversation. Her doctor is stunned, says Susan. Susan said her mother’s appearance has changed...”she looks and acts younger.” Note: Elderberries are high in anthocyanins- an anti-oxidant. Several persons have reported an anti-aging effect after using elderberry for 2 weeks or longer.

“V-Vax” Eucalyptus

Eucalyptus is found not only in Listerine, but in many brands of medicated cough drops. It is used in small quantities as it thought that large doses may be toxic. Eucalyptus is often used in saunas and in vaporizers and is helpful in clearing the sinuses and improving breathing through the lungs.

A former trappist monk, “Jack” told me of a report he heard from a veteran of World War I. He said that at an army barracks in Germany, there was an outbreak of meningitis and soldiers were dying of the affliction every day. One day, the army sealed off the barracks and sprayed the whole area with oil of eucalyptus and sealed all the windows. The soldiers inside were forced to breathe eucalyptus vapors all day. The veteran reported that the following day, the dying stopped and the epidemic ended.

Jack told me he believed that the vapors from the eucalyptus entered the blood of the soldiers via the lungs and killed the virus that was causing the meningitis. He also told me that he has been using 10 drops of V-Vax Eucalyptus daily for the past 10 years and has not had one single infection during this period. He said the species of eucalyptus used in V-Vax is non-toxic and comes from Australia. For more information, Jack can be reached at 773-276-1747.

References:

1. Efficacy of Listerine against MRSA, Candida Albicans and HIV, Yamanaka A et al, Bull Tokyo Dent Coll. 1994 Feb;35(1):23-6.

2. Antimicrobial evaluation of some plants used in Mexican traditional medicine...” Navarro V. et al, J Ethnopharmocol 1996 Sep;53(3):143-7

NATURAL COMBINATION THERAPIES

The Marc Correa protocol, available from Keep Hope Alive, is a good foundation protocol since it has been so successful.

Immune-based Therapies: See Th1 and Th2 Summary

Improving Macrophage and Neutrophil function; Beta 1, 3 Glucan. Use a high dose for 1 day per week (800 to 200 mg) or take a daily dose once in the morning upon rising (200 to 500 mg). Astragalus Jade Screen Formula (Planetary Formulas) - Take 2 to 4 tablets per day in the morning. If you use beta glucan and/or Jade Screen daily, then use NK911 in the evening for 3 days per week only. Note: Use small amounts of licorice root daily when using Jade Screen.

Glutathione replacement: In addition to supplements published in the current newsletter (Report No 17), be sure you are getting adequate amounts of Vitamin B12, Vitamin B6 and B2 (Riboflavin). B6 is needed to convert methionine to cysteine, the rate limiting amino acid in glutathione synthesis and Riboflavin helps to regenerate reduced glutathione.(1) B12 should be taken sublingually as it is not well absorbed in persons with either AIDS or CFIDS due to a deficiency of intrinsic factor. 1. Antioxidants and Functional Foods, Ronzio, Robert; Townsend Letter - December, 1998.

Nutritional Supplements - Individual - Cod Liver oil - 2 tablespoons daily, brewers yeast tablets - 9 twice daily, rose hips - may be used daily, calcium lactate (5 caps before bedtime) magnesium malate or magnesium oxide (500 mg daily), zinc sulfate (220 mg) - one tablet daily. Vitamin E - 200 to 400 i.u daily. Vitamin C - 4000 to 10,000 mg daily for 3 days per week and none for the 4 days in between. If you use NK911, use Vitamin C on the same days.

Medical Doctor reports that Venus Fly-trap extract eradicated Lyme’s disease in 6 weeks

Dr. Linley MD of Conncecticut wrote within the past month that she successfully treated herself for Lyme’s disease by taking Venus Fly-Trap extract 3 times a day for 6 weeks. After 6 weeks, all symptoms were gone and have not returned. She also strictly avoided sugar products which she says feed bacteria, fungus and viruses.

TRIPLE ESSENTIAL OIL WITH HONEY COMBO.

Heat 16 ounces of honey (about 1 and 1/2 cups) to a boil. Let cool. Add 1 teaspoon (tsp.) eucalyptus oil (V-Vax), 1 tsp. of peppermint oil and 1 tsp. orange oil and 2 tablespoons of lemon juice. Stir and refrigerate in a glass jar. Take 3 or 4 teaspoons daily. Based on scientific literature I have researched, this combo should kill 95 to 99% of all viral, fungal and bacterial infections it comes in contact with.

A 10 MONTH STUDY AT ST. LUKES-ROOSEVELT HOSPITAL WITH DIGESTIVE ENZYMES (WOBENZYM) FINDS THAT IT STABILIZED T CELL COUNTS AND VIRAL LOADS IN 21 HIV+ PATIENTS

At the 12th Int’l AIDS Conf, Geneva, Lange M and Maitra U et al report in abstract no We.B.3198, that Wobenzym, an oral enzyme preparation containing trypsin, chymotrypsin, papain, bromelain, pancreatin, lipase, amylase and the flavinoid, rutin. 21 HIV+ patients with a CD4 count between 200 and 500 used Wobenzym an average of 42 weeks or about 10 months. Tests were done to measure CD4 counts, PCR-Roche viral load and interluken-2 receptor (sCD25) and IFN-alpha. Conclusion: Wobenzym appeared to stabilize CD4 and HIV-RNA level in subjects..” The authors concluded that the use of Wobenzym alone or in combination with anti-virals should be further investigated.

SILICA - an anti-viral and immune booster

Silica is found in horsetail and in whole grain foods - the fiber portion. A book called “SILICA - THE AMAZING GEL, by Klaus Kaufman, reports on a case where silica gel applied topically cleared a herpes lesion in 5 days. The author also reports that silica has been used to successfully treat tuberculosis, warts, intestinal infections and increases immunity to cancer as well as stimulates phagocytes (macrophages and neutrophils) and lymphocytes (T cells). In Positive Health News, Report No 17, I reported on scientific research that indicates that silica decreases IgG, an immunoglobulin that when in excess diminishes Natural Killer cell activity.

Food sources of silica: Silica is found abundantly in the herb horsetail, millet, oats and the bran portion of whole grains and in onions and red beets. As a supplement, it is sold in health food stores as “Silica Gel.” A highly bio-available form of silica is Bio-Sil by Jarrow Formulas. Kaufman reports in his book that in Russia, a treatment for tuberculosis is to consume 20 grams (about 3/4 ounce) of pureed millet daily which provides 100 mg of soluble silica.

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